Probing Membrane Receptors with Enhanced Raman Imaging.
Probing Membrane Receptors with Enhanced Raman Imaging.
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使用增强拉曼成像探测膜受体。
DOI：
10.1117/12.2321300
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Schultz,ZacharyD
中科院分区：
文献类型：
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作者：
Xiao,Lifu;Sloan-Dennison,Sian;Schultz,ZacharyD
我们的实验室已经证明，用短肽功能化的纳米颗粒可以在体外选择性地结合受体蛋白。我们的研究结果表明，从纯化的受体在表面增强拉曼散射（SERS）实验中观察到的拉曼信号匹配与尖端增强拉曼散射（TERS）实验中观察到的完整细胞膜上的膜受体。对观察到的拉曼信号的分析表明，信号来自蛋白质受体中负责结合和识别附着于纳米颗粒探针的配体的氨基酸。进一步的实验表明，数据的变化与纳米颗粒探针与特定受体的亲和力相关。这一结果为研究膜受体提供了一种新的方法。
Our lab has shown that nanoparticles functionalized with short peptides can selectively bind to receptor proteins in vitro. Our results indicate that the Raman signals observed from purified receptors in surface enhanced Raman scattering (SERS) experiments match those observed with tip-enhanced Raman scattering (TERS) experiments performed on membrane receptors in intact cell membranes. Analysis of the observed Raman signals suggest the signals arise from the amino-acids in the protein receptor responsible for binding and recognition of the ligand attached to the nanoparticle probe. Further experiments show the variance in the data correlates with affinity of the nanoparticle probe with a specific receptor. This result illustrates a new approach to studying membrane receptors.