ENERGY TRANSDUCTION BY ANAEROBIC FERRIC IRON RESPIRATION IN THIOBACILLUS-FERROOXIDANS
ENERGY TRANSDUCTION BY ANAEROBIC FERRIC IRON RESPIRATION IN THIOBACILLUS-FERROOXIDANS
复制标题
DOI：
10.1128/aem.57.7.2063-2068.1991
复制
发表时间：
1991-07-01
影响因子：
4.4
通讯作者：
KUENEN, JG
中科院分区：
文献类型：
--
作者：
PRONK, JT;LIEM, K;KUENEN, JG
氧化亚铁硫杆菌（Thiobacillus ferrooxidans）是一种专性化化学化石自养嗜酸菌，在厌氧条件下能够进行甲酸和单质硫依赖的铁还原。在好氧条件下，氧和铁可以同时作为电子受体。为了研究氧化亚铁杆菌厌氧铁呼吸是否是一个能量转导过程，研究了氨基酸的摄取。在没有电子供体的情况下，无论是在有氧条件下还是在厌氧条件下，饥饿细胞都不会摄取甘氨酸。甘氨酸的摄取可以由甲酸和亚铁依赖的氧摄取驱动。在厌氧条件下，铁与电子供体甲酸酯和单质硫的呼吸作用可以激活甘氨酸的吸收，但解偶联剂2,4-二硝基苯酚抑制了它的作用。结果表明，厌氧铁呼吸有助于氧化亚铁杆菌的能量收支。
Formate-grown cells of the obligately chemolithoautotrophic acidophile Thiobacillus ferrooxidans were capable of formate- and elemental sulfur-dependent reduction of ferric iron under anaerobic conditions. Under aerobic conditions, both oxygen and ferric iron could be simultaneously used as electron acceptors. To investigate whether anaerobic ferric iron respiration by T. ferrooxidans is an energy-transducing process, uptake of amino acids was studied. Glycine uptake by starved cells did not occur in the absence of an electron donor, neither under aerobic conditions nor under anaerobic conditions. Uptake of glycine could be driven by formate- and ferrous iron-dependent oxygen uptake. Under anaerobic conditions, ferric iron respiration with the electron donors formate and elemental sulfur could energize glycine uptake was inhibited by the uncoupler 2,4-dinitrophenol. The results indicate that anaerobic ferric iron respiration can contribute to the energy budget of T. ferrooxidans.