Association of HLA-DRB1 and TNF genotypes with dengue hemorrhagic fever
Association of HLA-DRB1 and TNF genotypes with dengue hemorrhagic fever
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DOI：
10.1016/j.humimm.2013.01.027
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发表时间：
2013-05-01
期刊：
影响因子：
2.7
通讯作者：
Cecilia, D.
中科院分区：
文献类型：
--
作者：
Alagarasu, K.;Mulay, A. P.;Cecilia, D.
编码人类白细胞抗原（HLA）II类分子和肿瘤坏死因子（TNF）-α的基因是多态性的，并已被证明会影响感染性疾病的易感性。本研究应用PCR方法检测了114例登革患者（85例登革热（DF），29例登革出血热（DHF））和110例健康对照者（HC）的HLA-DRB 1、-DQB 1和TNF基因多态性。DHF组HLA-DRB 1 *07等位基因频率[DF vs. DHF，P = 0.0034，Pc = 0.044，OR 3.79]和HLA-DRB 1 *07/*15基因型频率[DF vs. DHF，P = 0.00071，Pc = 0.038，OR 10.411]显著高于HC组和DF组。DHF组rs 1800629“G/A”基因型频率高于DF组。TNF基因rs 1799964“C/C”基因型频率在所有患者组中均显著高于HC [HC vs. DEN，P = 0.0054，Pc = 0.0162，OR 3.57; HC vs. DF，P = 0.036，OR 2.89; HC与DHF，P = 0.0088，Pc = 0.0240，OR 5.11]。17.2%的DHF患者和1.2%的HC患者同时存在HLA-DRB 1 *07/*15基因型与rs 1799964“C/C”基因型或rs 1800629“G/A”基因型或两者同时存在，而DF患者中未观察到这种联合。结果表明，HLA-DRB 1 *07/*15基因型与TNF多态性的组合影响DHF的进展。(C)2013年美国组织相容性和免疫遗传学学会。爱思唯尔公司出版All rights reserved.
Genes coding for human leukocyte antigen (HLA) class II molecules and tumor necrosis factor (TNF)-alpha are polymorphic and have been shown to influence susceptibility to infectious diseases. In the present study, HLA-DRB1, -DQB1 and TNF gene polymorphisms were investigated in 114 dengue patients (DEN) [85 dengue fever (DF) cases and 29 dengue hemorrhagic fever (DHF) cases] and 110 healthy controls (HCs) using PCR based methods. The frequencies of HLA-DRB1*07 allele [DF vs. DHF, P = 0.0034, Pc = 0.044, OR 3.79] and HLA-DRB1*07/*15 genotype [DF vs. DHF, P = 0.00071, Pc = 0.038, OR 10.411 were significantly higher in DHF cases as compared to HCs and DF cases. Higher frequency of rs1800629 'G/A' genotype was observed in DHF cases as compared to DF cases. The frequency of rs1799964 'C/C' genotype of the TNF gene was found to be significantly higher in all patient groups compared to HCs [HCs vs. DEN, P = 0.0054, Pc = 0.0162, OR 3.57; HCs vs. DF, P = 0.036, OR 2.89; HCs vs. DHF, P = 0.0088, Pc = 0.0240, OR 5.11]. Presence of combination of HLA-DRB1*07/*15 with either rs1799964 'C/C' genotype or rs1800629 'G/A' genotype or both was present in 17.2% of DHF cases and 1.2% in HCs while this combination was not observed in DF cases. The results suggest that HLA-DRB1*07/*15 genotype in combination with TNF polymorphisms influence progression to DHF. (C) 2013 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved.