Chemokine-like factor-like MARVEL transmembrane domain containing 6: Bioinformatics and experiments in vitro analyze in glioblastoma multiforme.
Chemokine-like factor-like MARVEL transmembrane domain containing 6: Bioinformatics and experiments in vitro analyze in glioblastoma multiforme.
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DOI：
10.3389/fnmol.2022.1026927
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发表时间：
2022
影响因子：
4.8
通讯作者：
Qu, Yan
中科院分区：
文献类型：
--
作者：
Meng, Haining;Li, Shaohua;Li, Qingshu;Wang, Yuqin;Wang, Guoan;Qu, Yan
趋化因子样因子（Chemokine-like factor， CKLF）样MARVEL跨膜结构域6 （CMTM6）是一种定位于细胞膜上的蛋白，具有与质膜上PD-L1共定位、阻止PD-L1降解和维持细胞膜上PD-L1表达的能力。CMTM6在口腔鳞状细胞癌（OSCC）和结直肠癌（CRC）等多种肿瘤中高表达并发挥重要作用，但其在多形性胶质母细胞瘤（GBM）中的作用尚不清楚。为了探讨CMTM6在GBM中的作用，我们利用生物信息学方法分析了CMTM6在GBM中的表达、与CMTM6的相互作用及其相关基因。重要的是，我们分析了CMTM6在GBM中的表达与肿瘤浸润淋巴细胞（til）、免疫抑制剂、免疫刺激剂、趋化因子和趋化因子受体的关系。我们进一步分析了CMTM6的功能，并进行了体外实验验证。最后分析了CMTM6对药物的敏感性，并在体外验证了CMTM6与抗癌药物哌隆明（Piperlonguminine， PL）的关系。结果表明，CMTM6在GBM中高表达，且与多个基因相关。此外，CMTM6与GBM的免疫微环境和炎症反应密切相关。生物信息学分析CMTM6与GBM功能相关，我们的实验表明CMTM6显著促进GBM细胞的迁移和上皮间质转化（epithelial-mesenchymal transition， EMT），但对其他功能无显著影响。有趣的是，我们发现在GBM中，PL促进CMTM6的表达。在本文中，我们利用生物信息学分析和体外实验对CMTM6在GBM中的作用进行了详细的分析和验证，以证明CMTM6可能是胶质瘤治疗的潜在靶点。
Chemokine-like factor (CKLF)-like MARVEL transmembrane domain containing 6 (CMTM6) is a protein localized to the cell membrane and is known for its ability to co-localize with PD-L1 on the plasma membrane, prevent PD-L1 degradation, and maintain PD-L1 expression on the cell membrane. CMTM6 is highly expressed and plays an important role in various tumors such as oral squamous cell carcinoma (OSCC) and colorectal cancer (CRC), however, its role in Glioblastoma multiforme (GBM) is unclear. In this paper, to investigate the role of CMTM6 in GBM, we analyzed the expression of CMTM6 in GBM, the interaction with CMTM6 and the associated genes by bioinformatics. Importantly, we analyzed the expression of CMTM6 in GBM in relation to tumor-infiltrating lymphocytes (TILs), immunoinhibitors, immunostimulators, chemokines and chemokine receptors. We further analyzed the function of CMTM6 and performed in vitro experiments to verify it. Finally, the sensitivity of CMTM6 to drugs was also analyzed and the relationship between CMTM6 and the anticancer drug Piperlonguminine (PL) was verified in vitro. The results showed that CMTM6 was highly expressed in GBM and correlated with multiple genes. Furthermore, CMTM6 is closely related to the immune microenvironment and inflammatory response in GBM. Bioinformatic analysis of CMTM6 correlated with the function of GBM, and our experiments demonstrated that CMTM6 significantly promoted the migration of GBM cells and epithelial-mesenchymal transition (EMT), but had no significant effect on other functions. Interestingly, we found that in GBM, PL promotes the expression of CMTM6. In this paper, we have performed a detailed analysis and validation of the role of CMTM6 in GBM using bioinformatics analysis and in vitro experiments to demonstrate that CMTM6 may be a potential target for glioma therapy.
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影响因子：
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作者：
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期刊：
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影响因子：
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影响因子：
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作者：
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DOI：
10.1016/j.bbrc.2013.06.042
发表时间：
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影响因子：
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通讯作者：
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