Grooming analysis algorithm: Use in the relationship between sleep deprivation and anxiety-like behavior
Grooming analysis algorithm: Use in the relationship between sleep deprivation and anxiety-like behavior
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DOI：
10.1016/j.pnpbp.2012.11.006
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发表时间：
2013-03-05
影响因子：
5.6
通讯作者：
Andersen, Monica L.
中科院分区：
文献类型：
--
作者：
Pires, Gabriel N.;Tufik, Sergio;Andersen, Monica L.
焦虑增加是睡眠不足的典型后果。然而，关于睡眠剥夺引起的焦虑样行为的结果在啮齿动物模型中是矛盾的。梳理分析算法是一种基于梳理特征和微观结构来检查啮齿类动物的焦虑样行为和压力的方法。与传统的梳理分析相比，本研究评估了梳理分析算法区分睡眠剥夺和对照大鼠的适用性。 46 只动物被分为三组：对照组（n=22）、反常睡眠剥夺组（96 小时，n=10）和完全睡眠剥夺组（6 小时，n=14）。睡眠剥夺方案结束后，立即使用梳理分析算法和传统措施（梳理潜伏期、频率和持续时间）对梳理行为进行评估。结果表明，与对照组动物相比，矛盾睡眠剥夺组和完全睡眠剥夺组都在支离破碎的框架中表现出梳理行为。梳理分析算法的变量成功区分了睡眠不足和正常睡眠动物的焦虑样行为。修饰分析算法与传统措施密切相关。总之，梳理分析算法是评估焦虑类行为与睡眠剥夺之间关系的可靠方法。 (C) 2012 Elsevier Inc. 保留所有权利。
Increased anxiety is a classic effect of sleep deprivation. However, results regarding sleep deprivation-induced anxiety-like behavior are contradictory in rodent models. The grooming analysis algorithm is a method developed to examine anxiety-like behavior and stress in rodents, based on grooming characteristics and microstructure. This study evaluated the applicability of the grooming analysis algorithm to distinguish sleep-deprived and control rats in comparison to traditional grooming analysis. Forty-six animals were distributed into three groups: control (n=22), paradoxical sleep-deprived (96 h, n=10) and total sleep deprived (6 h, n=14). Immediately after the sleep deprivation protocol, grooming was evaluated using both the grooming analysis algorithm and traditional measures (grooming latency, frequency and duration). Results showed that both paradoxical sleep-deprived and total sleep-deprived groups displayed grooming in a fragmented framework when compared to control animals. Variables from the grooming analysis algorithm were successful in distinguishing sleep-deprived and normal sleep animals regarding anxiety-like behavior. The grooming analysis algorithm and traditional measures were strongly correlated. In conclusion, the grooming analysis algorithm is a reliable method to assess the relationship between anxiety-like behavior and sleep deprivation. (C) 2012 Elsevier Inc. All rights reserved.