Isolation and expression of a sterol carrier protein-2 gene from the yellow fever mosquito, Aedes aegypti
Isolation and expression of a sterol carrier protein-2 gene from the yellow fever mosquito, Aedes aegypti
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DOI：
10.1046/j.1365-2583.2003.00386.x
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发表时间：
2003-02-01
影响因子：
2.6
通讯作者：
Lan, Q
中科院分区：
文献类型：
--
作者：
Krebs, KC;Lan, Q
胆固醇在昆虫体内的运输是一个非常重要的过程，因为昆虫依赖于食物中的胆固醇来满足其生理需求。我们从4龄消减文库中克隆到一个可能的蚊虫甾醇携带蛋白-2(SCP-2)基因。AeSCP-2蛋白在类固醇转运域与大鼠和人的SCP-2具有高度的同源性。4龄幼虫AeSCP-2转录本在中肠表达较强，头部和后肠表达较弱。在早期蛹中，AeSCP-2在胸腔、头部和腹部的体壁中有转录，但在肠道中没有。用放射性标记胆固醇结合实验测得纯化的重组AeSCP-2对胆固醇的Kd为5.6+/-0.6×10(-9)m。这些结果表明，AeSCP-2对胆固醇有很高的亲和力，可能是蚊子体内的一种载体蛋白。
Trafficking of cholesterol in insects is a very important process due to the fact that insects depend on dietary cholesterol to fulfil their physiological needs. We identified a putative mosquito sterol carrier protein-2 (SCP-2) cDNA from fourth instar subtracted cDNA library. The AeSCP-2 protein has high degree homology in the sterol transfer domain to both rat and human SCP-2. Transcripts of AeSCP-2 in fourth instars were detected strongly in the midgut, and weakly in the head and hindgut. In the early pupae, AeSCP-2 transcription was observed in the thorax, head and body wall of abdomen, but not in the gut.The interaction of mosquito sterol carrier protein-2 (AeSCP-2) with cholesterol was examined. The K-d of purified recombinant AeSCP-2 to cholesterol was 5.6 +/- 0.6 x 10(-9) m using radiolabelled cholesterol-binding assay. The results suggest that AeSCP-2 has high affinity to cholesterol and may function as a carrier protein in mosquitoes.