Genetic analysis of mutants with a reduced Ca2+-dependent K+ current in Paramecium tetraurelia.
Genetic analysis of mutants with a reduced Ca2+-dependent K+ current in Paramecium tetraurelia.
复制标题
四尿草履虫中 Ca2 依赖性 K 电流降低的突变体的遗传分析。
DOI：
10.1093/genetics/111.3.433
复制
发表时间：
1985
期刊：
影响因子：
3.3
通讯作者：
Kung,C
中科院分区：
文献类型：
--
作者：
Hinrichsen,RD;Amberger,E;Saimi,Y;Burgess-Cassler,A;Kung,C
本文报道了四脲草履虫（Parameciumtetraurelia）的两个突变体，它们的Ca ~（2+）依赖性K ~+电流显著降低，并对其进行了遗传分析。这些突变体，命名为pantophobiac，比野生型细胞对所有刺激都产生更强的行为反应。在电压钳制下，这些突变体中的Ca 2+依赖的K+电流几乎完全消除，而Ca 2+电流是正常的。这两个突变体pntA和pntB是隐性的，彼此不连锁。四脲。显微注射的野生型细胞质的高速上清液部分到任一pantophobiac突变体引起暂时恢复到野生型表型。
Two mutants ofParamecium tetraureliawith greatly reduced Ca2+-dependent K+currents have been isolated and genetically analyzed. These mutants, designatedpantophobiac, give much stronger behavioral responses to all stimuli than do wild-type cells. Under voltage clamp, the Ca2+-dependent K+current is almost completely eliminated in these mutants, whereas the Ca2+current is normal. The two mutants,pntAandpntB, are recessive and unlinked to each other.pntAis not allelic to several other ion-channel mutants ofP. tetraurelia. The microinjection of a high-speed supernatant fraction of wild-type cytoplasm into either pantophobiac mutant caused a temporary restoration to the wild-type phenotype.