Effects of Terminalia arjuna bark extract on apoptosis of human hepatoma cell line HepG2
Effects of Terminalia arjuna bark extract on apoptosis of human hepatoma cell line HepG2
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DOI：
10.3748/wjg.v12.i7.1018
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发表时间：
2006-02-21
影响因子：
4.3
通讯作者：
Balasubramanian, Maruthaiveeran Periyasamy
中科院分区：
文献类型：
--
作者：
Sivalokanathan, Sarveswaran;Vijayababu, Marati Radhakrishnan;Balasubramanian, Maruthaiveeran Periyasamy
目的：探讨金龟子提取物对人肝癌细胞株(HepG2)的作用及其可能的诱导凋亡作用。方法：采用台盼蓝拒染法和乳酸脱氢酶漏出法测定不同浓度的金龟子乙醇提取物对人肝癌细胞株的细胞毒作用。光镜和荧光显微镜观察细胞凋亡，DNA片段化分析。通过P53和caspase-3蛋白的表达研究细胞凋亡的机制。结果：Arjua以浓度依赖的方式抑制HepG2细胞的增殖。60 mg/L和100 mg/L的Arjua作用于HepG2细胞后，可观察到细胞凋亡的形态，观察到DNA片段化、P53积聚和原aspase-3蛋白裂解。结论：金龟子对体外培养的人肝癌细胞具有明显的细胞毒作用。HepG2细胞的凋亡可能与DNA损伤和凋亡蛋白的表达有关。GSH耗竭可能参与了诱导HepG2细胞凋亡的过程。(C)2006年WJG出版社。版权所有。
AIM: To investigate the effects of Terminalia arjuna (T. arjuna) extract on human hepatoma cell line (HepG2) and its possible role in induction of apoptosis.METHODS: Human hepatoma cells were treated with different concentrations of ethanolic extract of T. arjuna and its cytotoxicity effect was measured by trypan blue exclusion method and lactate dehydrogenase leakage assay. Apoptosis was analyzed by light and fluorescence microscopic methods, and DNA fragmentation. The mechanism of apoptosis was studied with expression of p53 and caspase-3 proteins. Glutathione (GSH) content was also measured in HepG2 cells after T. arjuna treatment.RESULTS: T. arjuna inhibited the proliferation of HepG2 cells in a concentration-dependent manner. Apoptotic morphology was observed in HepG2 cells treated with T. arjuna at the concentrations of 60 and 100 mg/L. DNA fragmentation, accumulation of p53 and cleavage of procaspase-3 protein were observed in HepG2 cells after the treatment with T. arjuna. The depletion of GSH was observed in HepG2 cells treated with T. arjuna.CONCLUSION: T. arjuna induced cytotoxicity in HepG2 cells in vitro. Apoptosis of HepG2 cells may be due to the DNA damage and expression of apoptotic proteins. Depletion of GSH may be involved in the induction of apoptosis of HepG2 cells. (C) 2006 The WJG Press. All rights reserved.