The influence of presumptive limb connective tissue on motoneuron axon guidance.
The influence of presumptive limb connective tissue on motoneuron axon guidance.
复制标题
假定肢体结缔组织对运动神经元轴突引导的影响。
DOI：
10.1016/0012-1606(91)90057-a
复制
发表时间：
1991
影响因子：
2.7
通讯作者：
Dias,M
中科院分区：
文献类型：
--
作者：
Lance-Jones,C;Dias,M
在鸡的胚胎发生过程中，腰骶部（LS）的体胸膜产生结缔组织和四肢的表皮。我们希望确定LS体侧肋是否是沿着肢体前后轴运动神经元通路选择的指导线索的主要来源。在第15阶段，在肌细胞前体细胞和神经嵴形成之前，LS体侧膜向前移动。这种手术导致肢体的发展，转移到胸部区域一到四个部分。移位肢体的大腿前部的肌肉是正常的图案和复合来源：结缔组织的LS起源，而肌肉细胞的LS和胸部的起源。在ST 35-37的逆行HRP标记表明，这些大腿前肌的运动神经元池位于LS内，而不是胸髓段。单个肌肉的横截面积小于正常，但在LS索中占据的节段和横向位置与正常胚胎的横截面积大致匹配。这些发现表明，个别肌肉内躯体侧移位肢体的神经支配具体，并在雅阁其结缔组织（和表皮）的起源水平。在ST 28-31的神经模式的重建表明，LS运动神经元通过改变它们在大腿中部区域的通路来纠正这种移位。我们的结论是体侧板，最有可能是其结缔组织成分，包含的信息，建立一个特定的轴突指导系统在发展中的肢体。
During embryogenesis in the chick, the lumbosacral (LS) somatopleure gives rise to the connective tissue and the epidermis of the limb. We wished to determine if the LS somatopleure was a primary source of guidance cues for motoneuron pathway choices along the anteroposterior axis of the limb. At stage (st) 15, prior to its population by muscle cell precursors and the neural crest, the LS somatopleure was shifted anteriorly. This surgery resulted in the development of limbs that were shifted one to four segments into the thoracic region. Muscles within the anterior thigh of the shifted limb were normally patterned and of composite origin: connective tissues were of LS origin, while muscle cells were of LS and thoracic origin. Retrograde HRP labeling at st 35–37 indicated that motoneuron pools to these anterior thigh muscles were located within LS rather than thoracic cord segments. Pools to individual muscles were smaller than normal but occupied segmental and transverse positions in the LS cord that generally matched those of normal embryos. These findings suggest that individual muscles within somatopleure-shifted limbs are innervated specifically and are in accord with their connective tissue (and epidermal) level of origin. Reconstructions of nerve patterns at st 28–31 suggested that LS motoneurons corrected for the shift by altering their pathways at midthigh regions. We conclude that the somatopleure, and most likely its connective tissue component, contains the information for setting up a specific axon guidance system in the developing limb.