Histological and immunohistochemical evaluation of canine ovary
Histological and immunohistochemical evaluation of canine ovary
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DOI：
10.1111/j.1439-0531.2006.00812.x
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发表时间：
2007-10-01
影响因子：
1.7
通讯作者：
Taniyama, H.
中科院分区：
文献类型：
--
作者：
Akihara, Y.;Shimoyama, Y.;Taniyama, H.
本研究采用AE1/AE3、细胞角化蛋白7 （CK7）、CK13、CK20、vimentin、desmin、α -平滑肌肌动蛋白（α SMA）、钙钙蛋白、S100、神经丝、抑制素α、胎盘碱性磷酸酶（PLAP）和神经元特异性烯醇酶等大量蛋白进行组织学和免疫组织化学检查。卵巢结构包括表面上皮（SE）、皮质小管（CT）、白膜（TA）、间质细胞（SC）、内内分泌细胞（IE）、卵巢网（RO）和输卵管（FT）。SE、CT、RO和FT对desmin具有广泛的免疫反应性。除AE1/AE3和vimentin外，desmin也与这些结构密切相关。形成网状结构的网状卵巢对S100呈阳性反应。表面上皮对PLAP有明显高水平的免疫反应。综上所述，这些结果表明犬卵巢具有特定的免疫反应片段和广泛的免疫反应范围。即使在结构复杂的正常犬卵巢中，对各种蛋白质的免疫反应模式的不同也将对其识别和区分起重要作用。
In the present study, 15 canine ovaries without morphological lesions were examined histologically and immunohistochemically by using a large number of proteins including AE1/AE3, cytokeratin7 (CK7), CK13, CK20, vimentin, desmin, alpha smooth muscle actin (alpha SMA), calponin, S100, Neurofilaments, Inhibin alpha, placental alkaline phosphatase (PLAP) and neuron-specific enolase. Ovarian structures observed in this study included surface epithelium (SE), cortical tubules (CT), tunica albuginea (TA), stromal cells (SC), internal endocrine cells (IE), rete ovarii (RO) and fallopian tubes (FT). SE, CT, RO and FT were broadly immunoreactive for desmin. Besides AE1/AE3 and vimentin, desmin was also closely linked to these structures. Rete ovarii forming a reticular structure showed a positive reaction to S100. Surface epithelium was immunoreactive for PLAP at a significantly high level. In conclusion, these results indicate a specific segment of immunoreactivity as well as the broad range of immunoreactivity in canine ovary. The distinct patterns of immunoreactive for various kinds of proteins will play an important role in facilitating their identification and discrimination even in a normal canine ovary with a complex structure.