NUCLEOTIDE-SEQUENCE OF THE MATRIX, FUSION AND PUTATIVE SH PROTEIN GENES OF MUMPS-VIRUS AND THEIR DEDUCED AMINO-ACID SEQUENCES
NUCLEOTIDE-SEQUENCE OF THE MATRIX, FUSION AND PUTATIVE SH PROTEIN GENES OF MUMPS-VIRUS AND THEIR DEDUCED AMINO-ACID SEQUENCES
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DOI：
10.1016/0168-1702(89)90054-3
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发表时间：
1989-01-01
期刊：
影响因子：
5
通讯作者：
RIMA, BK
中科院分区：
文献类型：
--
作者：
ELLIOTT, GD;AFZAL, MA;RIMA, BK
已经制备了代表腮腺炎病毒SBL株的M、F和推定SH基因的cDNA克隆并测定了它们的核苷酸序列。腮腺炎病毒的 M 基因似乎包含 1253 个核苷酸，编码 375 个氨基酸残基的蛋白质 (Mr 41 589)。该蛋白质是疏水性的，推导的氨基酸序列与其他副粘病毒的氨基酸序列具有同源性。将 SBL 菌株的 F 基因与 RW 菌株的 F 基因进行比较 [Waxham 等人。 (1987) 病毒学 159, 381-388]。 F基因长1727个核苷酸，编码538个氨基酸的蛋白质（Mr 58 789）。不同腮腺炎病毒株的F基因序列之间存在显着差异。 F 基因后面是一个小的（315 nt）转录单位，其中包含一个由 57 个氨基酸组成的开放阅读框，编码一种非常疏水的蛋白质（Mr 6712）。这可能与 SV5 的 SH 基因相似，尽管 SV5 SH 蛋白和推定的腮腺炎病毒 SH 蛋白之间似乎没有序列同源性。腮腺炎病毒的物理和转录图谱显示其基因顺序为3''-N-P-M-F-SH-HN，与其他副粘病毒尤其是SV5相似。
cDNA clones representing the M, F and a putative SH gene of the SBL strain of mumps virus have been prepared and their nucleotide sequence determined. The M gene of mumps virus appears to contain 1253 nucleotides and codes for a protein of 375 amino acid residues (Mr 41 589). The protein is hydrophobic and the deduced amino acid sequence shows homologies with those of other paramyxoviruses. The F gene of the SBL strain was compared to that of the RW strain [Waxham et al. (1987) Virology 159, 381-388]. The F gene is 1727 nucleotides long and codes for a protein of 538 amino acids (Mr 58 789). There are substantial variations between the F gene sequences of various mumps virus strains. The F gene is followed by a small (315 nt) transcription unit which contains an open reading frame of 57 amino acids encoding a very hydrophobic protein (Mr 6712). This may be similar to the SH gene of SV5, although there appears to be no sequence homology between the SV5 SH protein and the putative SH protein of mumps virus. A physical and transcription map of mumps virus indicates the gene order to be 3''-N-P-M-F-SH-HN, similar to that of other paramyxoviruses and SV5 in particular.