SPIROPLASMA IXODETIS SP-NOV, A NEW SPECIES FROM IXODES PACIFICUS TICKS COLLECTED IN OREGON
SPIROPLASMA IXODETIS SP-NOV, A NEW SPECIES FROM IXODES PACIFICUS TICKS COLLECTED IN OREGON
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DOI：
10.1099/00207713-45-1-23
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发表时间：
1995-01-01
期刊：
影响因子：
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通讯作者：
WHITCOMB, RF
中科院分区：
文献类型：
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作者：
TULLY, JG;ROSE, DL;WHITCOMB, RF
从从俄勒冈州采集的西部黑腿硬蜱制备的混合悬浮液中分离到8株软体软体动物。电子显微镜和暗场显微镜观察表明，每个菌株均由可移动的紧密盘绕的螺旋细胞、直径为300~500 nm的小球状细胞和多形性、直丝状或分枝状丝状细胞组成。所有的细胞型都被单一的细胞膜包围，没有细胞壁的证据。这些生物是可过滤的，在生长要求上也很挑剔。生长的最适温度为30℃，但在23-32℃之间发生增殖。该菌株分解葡萄糖，但不降解精氨酸或尿素。Y32(T)株(T=模式株)基因组大小为2220kbp，DNA碱基组成(鸟嘌呤+胞嘧啶含量)为25+/-1摩尔%。这8个分离株在血清学上彼此相关，但与属于螺旋体属的37个其他类型或代表性菌株没有关系。菌株Y32(=atcc 33835)是硬毛螺旋体的模式菌株。11月
Eight strains of mollicutes were isolated from pooled suspensions prepared from western black-legged ticks (Ixodes pacificus) collected in Oregon. Morphologic examination by electron and dark-field microscopic techniques showed that each strain consisted of a mixture of motile, tightly coiled helical cells, small coccoid cells with diameters ranging from 300 to 500 nm, and pleomorphic, straight or branched filamentous forms. All cellular forms were surrounded by a single cytoplasmic membrane, and there was no evidence of a cell wall. The organisms were filterable and fastidious in their growth requirements. The optimum temperature for growth was 30 degrees C, but multiplication occurred at temperatures ranging from 23 to 32 degrees C. The strains catabolized glucose but did not hydrolyze arginine or urea. The genome size of strain Y32(T) (T = type strain) was 2,220 kbp, and the DNA base composition (guanine-plus-cytosine content) of this organism was 25 +/- 1 mol%. The eight isolates were serologically related to each other but were not related to 37 other type or representative strains belonging to the genus Spiroplasma. Strain Y32 (= ATCC 33835) is the type strain of Spiroplasma ixodetis sp. nov.