Regulation of CLV 3 Expression by Two Homeobox Genes in Arabidopsis 1
Regulation of CLV 3 Expression by Two Homeobox Genes in Arabidopsis 1
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发表时间：
2002
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通讯作者：
U. Brand;M. Grünewald;M. Hobe;R. Simon
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作者：
U. Brand;M. Grünewald;M. Hobe;R. Simon
分生组织持续产生新器官的能力取决于位于分生组织顶端的干细胞群的活性。在拟南芥中，干细胞结构域的大小由两种拮抗活性调节。WUS（WUSCHEL）基因编码同源结构域蛋白，促进干细胞的形成和维持。这些干细胞表达CLV 3（CLAVATA 3），并且CLV 3通过CLV 1/CLV 2受体复合物的信号传导限制WUS活性。干细胞群体的稳态可通过反馈调节实现，由此干细胞数量的变化导致CLV 3表达水平的相应变化，并通过CLV信号转导途径调节WUS表达。我们分析了CLV 3的表达是否受WUS或另一种同源框基因STM（SHOOT MERISTEMLESS）的活性控制，这是干细胞维持所必需的。我们发现CLV 3的表达仅依赖于胚茎分生组织中的WUS功能。在后期发育阶段，WUS促进CLV 3的表达水平，连同STM。在分生组织内，通过表达CLV 3响应WUS活性的能力限于分生组织顶端。
The ability of meristems to continuously produce new organs depends on the activity of their stem cell populations, which are located at the meristem tip. In Arabidopsis, the size of the stem cell domain is regulated by two antagonistic activities. The WUS (WUSCHEL) gene, encoding a homeodomain protein, promotes the formation and maintenance of stem cells. These stem cells express CLV3 (CLAVATA3), and signaling of CLV3 through the CLV1/CLV2 receptor complex restricts WUS activity. Homeostasis of the stem cell population may be achieved through feedback regulation, whereby changes in stem cell number result in corresponding changes in CLV3 expression levels, and adjustment of WUS expression via the CLV signal transduction pathway. We have analyzed whether expression of CLV3 is controlled by the activity of WUS or another homeobox gene, STM (SHOOT MERISTEMLESS), which is required for stem cell maintenance. We found that expression of CLV3 depends on WUS function only in the embryonic shoot meristem. At later developmental stages, WUS promotes the level of CLV3 expression, together with STM. Within a meristem, competence to respond to WUS activity by expressing CLV3 is restricted to the meristem apex.