THE OVALBUMIN GENE IS ASSOCIATED WITH THE NUCLEAR MATRIX OF CHICKEN OVIDUCT CELLS
THE OVALBUMIN GENE IS ASSOCIATED WITH THE NUCLEAR MATRIX OF CHICKEN OVIDUCT CELLS
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DOI：
10.1016/0092-8674(82)90379-8
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发表时间：
1982-01-01
期刊：
影响因子：
64.5
通讯作者：
VOGELSTEIN, B
中科院分区：
文献类型：
--
作者：
ROBINSON, SI;NELKIN, BD;VOGELSTEIN, B
真核生物细胞核中的DNA被排列成一系列超螺旋环，这些环在其基部锚定到核基质上。核酸酶消化可以逐渐从环切割DNA，从而分离逐渐接近核基质锚定位点的残留DNA。卵清蛋白基因优先与鸡输卵管细胞的核基质相关，但不优先与鸡肝细胞的核基质相关。作为对照，珠蛋白基因在输卵管细胞中不转录，被发现不优先与输卵管核基质结合。转录活性卵清蛋白基因优先与核基质相关的观察可能对基因表达和核DNA组织成超卷曲环结构域具有重要意义。
The DNA in a eukaryotic nucleus is arranged into a series of supercoiled loops that are anchored at their bases to the nuclear matrix. Nuclease digestion can progressively cleave DNA from the loops, thereby isolating residual DNA that is progressively closer to the nuclear matrix anchorage sites. The ovalbumin gene is preferentially associated with the nuclear matrix of chicken oviduct cells, but is not preferentially associated with the nuclear matrix of chicken liver cells. As a control, the .beta.-globin gene, which is not transcribed in oviduct cells, was found not to be preferentially associated with the oviduct nuclear matrix. The observation that the transcriptionally active ovalbumin gene is preferentially associated with the nuclear matrix may have significant implications for gene expression and the organization of nuclear DNA into supercoiled-loop domains.