Puerarin alleviates radicular pain caused by lumbar disc herniation by inhibiting spinal glial cell activation and inflammatory response
Puerarin alleviates radicular pain caused by lumbar disc herniation by inhibiting spinal glial cell activation and inflammatory response
复制标题
葛根素通过抑制脊髓胶质细胞活化和炎症反应减轻腰椎间盘突出引起的根性疼痛
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发表时间：
2019
期刊：
影响因子：
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通讯作者：
ZHONG Yi
中科院分区：
文献类型：
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作者：
ZHONG Yi
AIM： To investigate the role of Chinese medical herb Radix Puerariae extract puerarin in a rat model.of radicular pain caused by lumbar disc herniation （RAPLDH） and to explore the possible mechanism involving spinal glial.cell activation and inflammatory response.METHODS： The rat model of RAPLDH was induced by autologous nucleus pul －.posus （NP） implantation.The rats in sham group received the same operation procedure except NP implantation .Puerarin.injection at different doses （50， 100 and 150 mg／kg） was delivered intraperitoneally 1 h before surgery， and once daily for.7 d.Mechanical paw withdrawal threshold （PWT） test was employed for assessing pain behaviors .Spinal microglia and astrocyte.activation was evaluated by immunofluorescence staining of relevant specific markers .The expression of pro－and antiinflammatory.cytokines in spinal dorsal horn was measured by ELISA .RESULTS： The rats with NP implantation showed.long－lasting pain behaviors， characterized by decrease in PWT from day 3 to day 14 after surgery.Compared with vehicle.group， puerarin at doses of 100 mg／kg and 150 mg／kg significantly increased PWT of the rats with NP implantation .Puerarin.significantly reduced the expression of spinal microglia marker ionized calcium －binding adaptor molecule 1 and astrocyte.marker glial fibrillary acidic protein （P ＜0.01）.Puerarin also decreased spinal expression of pro －inflammatory cytokines.tumor necrosis factor－α， interleukin－1βand interleukin－6， and increased anti－inflammatory cytokine interleukin－10 （P ＜.0.01）.CONCLUSION： Puerarin alleviate RAPLDH by inhibiting spinal glial cell activation and inflammatory response
