Proliferation patterns of dorsal root ganglion neurons of cutaneous, muscle and visceral nerves in the rat
Proliferation patterns of dorsal root ganglion neurons of cutaneous, muscle and visceral nerves in the rat
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DOI：
10.1023/a:1025760116189
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发表时间：
2002-09-01
期刊：
影响因子：
--
通讯作者：
Grant, G
中科院分区：
文献类型：
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作者：
Kitao, Y;Robertson, B;Grant, G
在之前的一项研究中，我们提供的证据表明不同表型的背根神经节（DRG）神经元具有不同的出生日期。本研究旨在确定 DRG 神经元的出生日期是否与不同类型的周围神经相关，即皮肤与肌肉、体细胞与内脏神经。怀孕大鼠腹膜内注射溴脱氧尿苷 (BrdU)，以在胚胎 E12-E16 之一标记神经元。当子代大鼠成年时，将1%霍乱毒素B片段和1%加纳单叶异凝集素B4的混合物注射到周围神经中，或将5%氟金溶液涂抹在神经横断端。隐神经和腓肠神经用作皮神经，腓肠肌神经用作肌神经，肋间神经T9-11用作体神经，内脏大神经用作内脏神经。对不同胚胎阶段的两个皮神经和肌肉神经标记的 DRG 神经元以及用 BrdU 标记的隐神经和腓肠肌神经元进行细胞大小测量。肌肉和肋间神经的 DRG 神经元大部分生成较早，峰值出现在 E13，而皮肤和内脏传入神经的 DRG 神经元生成较晚，峰值出现在 E14。时间差异反映在细胞大小谱中，与皮神经相比，肌肉神经具有更大比例的大神经元。这些发现补充了先前关于不同 DRG 表型发育顺序的知识。
In a previous study we provided evidence that dorsal root ganglion (DRG) neurons of different phenotypes have different birthdates. The present study aimed at determining if birthdates of DRG neurons are related to different types of peripheral nerves, namely cutaneous versus muscle, and somatic versus visceral. Pregnant rats were injected intraperitoneally with bromodeoxyuridine (BrdU) to label the neurons on one of the embryonic days E12-E16. When the progeny rats reached adulthood, a mixture of 1% B-fragment of cholera toxin and 1% isolectin B4 from Griffonia simplicifolia I was injected into the peripheral nerves, or a 5% Fluoro-Gold solution was applied to the transected end of the nerves. The saphenous and sural nerves were used as cutaneous nerves, the gastrocnemius nerve as a muscle nerve, the intercostal nerves T9-11 as somatic nerves and the greater splanchnic nerve as a visceral nerve. Cell size measurements were made of DRG neurons labeled from the two cutaneous nerves and the muscle nerve, as well as of neurons of the saphenous and gastrocnemius nerves labeled by BrdU at different embryonic stages. Most of the DRG neurons of the muscle and intercostal nerves were generated early, with peaks at E13, and those of the cutaneous and visceral afferent nerves later, with peaks at E14. The temporal differences were reflected in the cell size spectrum, the muscle nerve having a greater proportion of large neurons compared to the cutaneous nerves. The findings add to previous knowledge regarding the sequence of development of different DRG phenotypes.