SELF-ASSEMBLED ORGANIC MONOLAYERS - MODEL SYSTEMS FOR STUDYING ADSORPTION OF PROTEINS AT SURFACES
SELF-ASSEMBLED ORGANIC MONOLAYERS - MODEL SYSTEMS FOR STUDYING ADSORPTION OF PROTEINS AT SURFACES
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DOI：
10.1126/science.252.5009.1164
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发表时间：
1991-05-24
期刊：
影响因子：
56.9
通讯作者：
WHITESIDES, GM
中科院分区：
文献类型：
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作者：
PRIME, KL;WHITESIDES, GM
欧米伽功能化长链硫代烷基硫酸酯在金膜上的自组装单分子膜(SAM)是研究蛋白质与有机表面相互作用的良好模型体系。含有疏水性(甲基封端的)和亲水性的[羟基、麦芽糖和六(乙二醇)封端的]烷硫醇混合物的单分子膜可以定制以选择特定的吸附程度：吸附的蛋白质量随单分子层的组成而单调变化。六(乙二醇基)封端的自组装膜在抵抗蛋白质吸附方面最有效。创建具有相似结构和定义良好的组成的界面的能力应该使测试关于蛋白质吸附的假设成为可能。
Self-assembled monolayers (SAMs) of omega-functionalized long-chain alkanethiolates on gold films are excellent model systems with which to study the interactions of proteins with organic surfaces. Monolayers containing mixtures of hydrophobic (methyl-terminated) and hydrophilic [hydroxyl-, maltose-, and hexa(ethylene glycol)-terminated] alkanethiols can be tailored to select specific degrees of adsorption: the amount of protein adsorbed varies monotonically with the composition of the monolayer. The hexa(ethylene glycol)-terminated SAMs are the most effective in resisting protein adsorption. The ability to create interfaces with similar structures and well-defined compositions should make it possible to test hypotheses concerning protein adsorption.