BIOCHEMICAL-CHARACTERIZATION OF A PUTATIVE AXONAL GUIDANCE MOLECULE OF THE CHICK VISUAL-SYSTEM
BIOCHEMICAL-CHARACTERIZATION OF A PUTATIVE AXONAL GUIDANCE MOLECULE OF THE CHICK VISUAL-SYSTEM
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DOI：
10.1016/0896-6273(90)90227-7
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发表时间：
1990-11-01
期刊：
影响因子：
16.2
通讯作者：
BONHOEFFER, F
中科院分区：
文献类型：
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作者：
STAHL, B;MULLER, B;BONHOEFFER, F
在顶盖膜地毯上生长的离体颞叶视网膜轴突被后顶盖细胞膜偏转。负责这种偏转的活性可以被针对顶盖膜和相应的Fab片段产生的抗体消除。顶盖膜的二维凝胶电泳和免疫印迹分析揭示了一个33 kD的糖蛋白，具有较高的浓度在后比在前顶盖。其表达受发育调节，对磷脂酰肌醇特异性磷脂酶C敏感。这些都是预期的分子负责在视网膜轴突的体外指导实验中观察到的现象的属性。
Temporal retinal axons growing in vitro on carpets of tectal membranes are deflected by cell membranes of posterior tectum. The activity responsible for this deflection can be abolished by antibodies raised against tectal membranes and the corresponding Fab fragments. Analysis of tectal membranes by two-dimensional gel electrophoresis and immunoblotting reveals a 33 kd glycoprotein that has a higher concentration in posterior than in anterior tectum. Its expression is developmentally regulated, and it is sensitive to phosphatidylinositol-specific phospholipase C. These are properties expected for a molecule responsible for the phenomena observed in experiments on in vitro guidance of retinal axons.