Evaluation of a Modular Multiplex-PCR Methicillin-Resistant Staphylococcus aureus Detection Assay Adapted for mecC Detection

Evaluation of a Modular Multiplex-PCR Methicillin-Resistant Staphylococcus aureus Detection Assay Adapted for mecC Detection
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DOI:
10.1128/jcm.00075-13
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发表时间:
2013-06-01
影响因子:
9.4
通讯作者:
Kriegeskorte, Andre
Kriegeskorte, Andre
中科院分区:
医学2区
文献类型:
--
作者:
Becker, Karsten;Larsen, Anders R.;Kriegeskorte, Andre

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使用国际 spa 型金黄色葡萄球菌集合(包括 51 个 mecC 阳性 MRSA、240 个 mecA 阳性 MRSA 和 50 个 mecA 和 mecC 阴性)对基于 mecC (mecA(LGA251)) 的多重 PCR 耐甲氧西林金黄色葡萄球菌 (MRSA) 检测分析进行了评估 甲氧西林敏感金黄色葡萄球菌 (MSSA) 分离株。该测定对金黄色葡萄球菌物种鉴定以及 mecA 和 mecC 检测显示出 100% 的灵敏度和特异性。
A mecC (mecA(LGA251))-adapted multiplex PCR-based methicillin-resistant Staphylococcus aureus (MRSA) detection assay was evaluated using an international, spa-typed Staphylococcus aureus collection comprising 51 mecC-positive MRSA, 240 mecA-positive MRSA, and 50 mecA-and mecC-negative methicillin-susceptible S. aureus (MSSA) isolates. The assay showed 100% sensitivity and specificity for S. aureus species identification as well as for mecA and mecC detection.