A Study on the Mechanism of DNA Excretion from P. aeruginosa KYU-1
A Study on the Mechanism of DNA Excretion from P. aeruginosa KYU-1
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铜绿假单胞菌KYU-1排泄DNA机制的研究
DOI：
10.1271/bbb1961.45.2457
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
S. Ueda
中科院分区：
文献类型：
--
作者：
T. Hara;S. Ueda
利用KYU-1菌株对DNA的排泄机制进行了研究。该菌株胞外DNA产量相当高(7.8 mg/ml)，每毫升细胞外DNA产量是每毫升细胞内DNA产量的数十倍。加入抑制剂(奈利地酸、5-氟尿嘧啶或丝裂霉素C)可抑制DNA合成，抑制率为35%~54%。在对数生长期中期加入10 0μg/ml的丝裂霉素C，每毫升细胞只能获得0.89 mg的胞外DNA，抑制率为95%。然而，无论有无抑制剂，在培养中合成的有机磷的量几乎是相同的。此外，用电子显微镜观察了菌株Kyu-1细胞表面的DNA排泄情况，发现排泄的DNA分子是双链的。
The mechanism of excretion of DNA was investigated using strain KYU-1. This strain produced a considerable amount of DNA extracellularly (7.8 mg/ml), and the amount of extracellular DNA per ml of the culture was scores of times more compared to that of intracellular DNA per ml of the culture. DNA synthesis was repressed by the addition of inhibitors (nalidixic acid, 5-fluoro uracil or mitomycin C), their inhibition rates were 35 to 54%. With the addition of 100 μg/ml of mitomycin C at the middle of the logarithmic growth phase, only 0.89 mg of extracellular DNA per ml of the culture was obtained, the inhibition rate was 95%. However, the amount of organic phosphate synthesized in the culture was almost the sameno matter whether with or without an inhibitor. Furthermore, the excretion of DNA from the cell surface of strain KYU-1 was observed with on electron microscope and the DNA molecules excreted were found to be double-stranded.