In vitro periodontal ligament fibroblast attachment to plasma-cleaned titanium surfaces.
In vitro periodontal ligament fibroblast attachment to plasma-cleaned titanium surfaces.
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发表时间：
1991
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通讯作者：
C. M. Michaels;J. Keller;C. Stanford
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作者：
C. M. Michaels;J. Keller;C. Stanford
本研究的目的是表征来自大鼠牙周膜的成纤维细胞样细胞在商业纯钛表面上的体外细胞行为，所述商业纯钛表面通过各种处理灭菌。按照标准表面制备方案，通过蒸汽高压灭菌、暴露于环氧乙烷气体、暴露于紫外线或氩气中等离子体清洁对钛样本进行灭菌1 min或5 min。将含血清培养基中的成纤维细胞样细胞在各种钛样本上孵育2 h。一般来说，等离子体清洁表面的细胞附着水平显著高于蒸汽灭菌表面，但显著低于紫外线处理表面和组织培养塑料对照的附着水平。等离子体清洁本身的持续时间在任何时间段对细胞附着的百分比没有显著影响。SEM评价表明，通过两个小时，细胞形态是不同的各种处理的标本。这些研究表明，植入物表面制备后的灭菌方法可影响细胞附着和扩散的初始体外生物学事件。
The objective of this research was to characterize the in vitro cellular behavior of fibroblast-like cells derived from rat periodontal ligament on commercially pure titanium surfaces which were sterilized by a variety of treatments. Following standard surface preparation protocols, the Ti specimens were sterilized by either steam autoclaving, exposure to ethylene oxide gas, exposure to ultraviolet light, or plasma-cleaning in argon for either one min or five min. Fibroblast-like cells in serum-supplemented media were incubated on the various Ti specimens for up to two h. In general, the levels of cell attachment for plasma-cleaned surfaces were significantly higher than those for steam-sterilized surfaces, but were significantly lower than the attachment levels for both the ultraviolet-treated surfaces and the tissue culture plastic control. The duration of plasma cleaning itself did not have a significant effect on the percentage of cell attachment at any time period. SEM evaluations indicated that by two h, the cellular morphology was different on the variously treated specimens. These studies indicate that the method of sterilization following implant surface preparation can affect the initial in vitro biological events of cell attachment and spreading.