Increased seed cytokinin levels in transgenic tobacco influence embryo and seedling development.
Increased seed cytokinin levels in transgenic tobacco influence embryo and seedling development.
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DOI：
10.1071/pp01211
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发表时间：
2002-08
期刊：
影响因子：
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通讯作者：
Q. Ma;Zhan-Bing Lin;D. Fu
中科院分区：
文献类型：
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作者：
Q. Ma;Zhan-Bing Lin;D. Fu
异源豌豆毒蛋白异戊烯基转移酶（ipt）基因的种子特异性表达已在转基因烟草品系7和16中确定。自花受精后代的遗传分析表明，一个单一拷贝的Vicilin-ipt基因已被整合，和T2后代已成为纯合的转基因。聚合酶链反应分析证实了Vicilin-ipt基因在T3代中的稳定遗传。种子细胞分裂素水平比对照水平高2-3倍。细胞学分析显示，与对照相比，转基因胚胎的染色体细胞层数和直径显著增加。成熟种子的干重和随后的幼苗生长显着增加。这与转基因烟草种子中豌豆毒蛋白-ipt过表达水平和细胞分裂素水平增加有关。结果表明，细胞分裂素在烟草胚发育的调控中起着重要作用。
Seed-specific expression of a heterologous vicilin-isopentenyl transferase (ipt) gene has been determined in transgenic tobacco lines 7 and 16. Genetic analysis of self-fertilized progeny showed that a single copy of the vicilin-ipt gene had been integrated, and T2 progeny had become homozygous for the transgene. Stable inheritance of the vicilin-ipt gene in T3 progeny was verified by polymerase chain reaction analysis. Seed cytokinin levels were 2-3-fold higher than control levels. Cytological analyses revealed a significant increase in the number of plerome cell layers and enlargement of diameter in transgenic embryos compared with controls. Dry weight of mature seeds and subsequent seedling growth was increased significantly. This correlated with the level of vicilin-ipt overexpression and increased cytokinin levels in transgenic tobacco seeds. The results suggest a crucial role for cytokinins in regulation of tobacco embryo development.