De novo transcriptome sequencing and comparative analysis of midgut tissues of four non-model insects pertaining to Hemiptera, Coleoptera, Diptera and Lepidoptera
De novo transcriptome sequencing and comparative analysis of midgut tissues of four non-model insects pertaining to Hemiptera, Coleoptera, Diptera and Lepidoptera
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DOI：
10.1016/j.gene.2017.06.008
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发表时间：
2017-09-05
期刊：
影响因子：
3.5
通讯作者：
Venancio, Thiago M.
中科院分区：
文献类型：
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作者：
Gazara, Rajesh K.;Cardoso, Christiane;Venancio, Thiago M.
昆虫的形态多样，但其消化功能却有一定的规律性，这显然与昆虫的生理有关。在目前的工作中，我们报告了从头中肠转录组的四个非模式昆虫从四个不同的目的：草地贪夜蛾（鳞翅目），家蝇（双翅目），黄粉虫（鞘翅目）和秘鲁披碱蜂（半翅目）。我们采用了一种计算策略来合并用两种不同算法获得的组件，这大大提高了最终转录组的质量。使用eggNOG数据库对Unigenes进行了注释和分析，这使我们能够为79.7%至93.1%的转录组分配一定水平的功能和进化信息。我们发现有趣的转录模式，如：i）强烈使用溶菌酶的消化功能的M。ii）在不同物种的整个中肠中，正磷酸尿苷-糖基转移酶和细胞色素P450基因的上调，支持了对抗异生物质的古老防御前线的存在; iii）保幼激素结合蛋白在中肠生理学中的证据支持作用，可能是作为一种激活基因的方式，帮助对抗抗营养物质（例如蛋白酶抑制剂）。这里提出的结果揭示了这些远亲物种的消化系统的消化和结构特性。此外，所产生的数据集也将对研究这些昆虫的科学家有用。
Despite the great morphological diversity of insects, there is a regularity in their digestive functions, which is apparently related to their physiology. In the present work we report the de novo midgut transcriptomes of four non-model insects from four distinct orders: Spodoptera frugiperda (Lepidoptera), Musca domestica (Diptera), Tenebrio molitor (Coleoptera) and Dysdercus peruvianus (Hemiptera). We employed a computational strategy to merge assemblies obtained with two different algorithms, which substantially increased the quality of the final transcriptomes. Unigenes were annotated and analyzed using the eggNOG database, which allowed us to assign some level of functional and evolutionary information to 79.7% to 93.1% of the transcriptomes. We found interesting transcriptional patterns, such as: i) the intense use of lysozymes in digestive functions of M. domestica larvae, which are streamlined and adapted to feed on bacteria; ii) the up-regulation of orthologous UDP-glycosyl transferase and cytochrome P450 genes in the whole midguts different species, supporting the existence of an ancient defense frontline to counter xenobiotics; iii) evidence supporting roles for juvenile hormone binding proteins in the midgut physiology, probably as a way to activate genes that help fight anti-nutritional substances (e.g. protease inhibitors). The results presented here shed light on the digestive and structural properties of the digestive systems of these distantly related species. Furthermore, the produced datasets will also be useful for scientists studying these insects.