Differential cellular gene expression induced by hepatitis B and C viruses
Differential cellular gene expression induced by hepatitis B and C viruses
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DOI：
10.1016/s0006-291x(02)02861-9
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发表时间：
2003-01-10
影响因子：
3.1
通讯作者：
Seki, N
中科院分区：
文献类型：
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作者：
Otsuka, M;Aizaki, H;Seki, N
B型肝炎病毒（HBV）是一种嗜肝病毒，可引起急性和慢性肝细胞损伤和肝细胞癌。为了阐明HBV蛋白如何调节宿主细胞基因表达，我们使用了我们的内部cDNA微阵列和HepG2.2.15细胞，它们来自HepG2细胞并产生所有HBV蛋白。在2304个基因中，有几个基因在HepG2.2.15细胞中与HepG2细胞相比差异表达。这些基因包括胰岛素样生长因子II和甲胎蛋白，与以前的报告一致。此外。我们先前使用产生所有HCV蛋白的HepG2衍生细胞系进行了类似的微阵列分析，以阐明丙型肝炎病毒（HCV）蛋白对宿主细胞的影响。利用这两个微阵列结果，我们比较了HBV和HCV蛋白诱导的细胞基因表达的差异。研究的大多数基因的表达在HBV和HCV蛋白生产细胞之间仅略有不同。然而，HBV和HCV蛋白以相互的方式明显调节几个基因。结合起来，这些微阵列结果揭示了新的光HBV蛋白对细胞基因表达的影响和这两种肝炎病毒的致病活性的差异。(C)2002 Elsevier Science（美国）。All rights reserved.
Hepatitis B virus (HBV) is a hepatotropic virus that causes acute and chronic hepatocellular injury and hepatocellular carcinoma. To clarify how HBV proteins regulate host cellular gene expression, we used our in-house cDNA microarray and HepG2.2.15 cells, which are derived from HepG2 cells and produce all HBV proteins. Of 2304 genes investigated, several genes were differentially expressed in HepG2.2.15 cells compared with HepG2 cells. These genes included insulin-like growth factor II and alpha-fetoprotein, consistent with previous reports. Furthermore. we previously performed similar microarray analyses to clarify the effects of hepatitis C virus (HCV) proteins on host cells, using a HepG2-derivative cell line, which produces all HCV proteins. Using these two microarray results, we compared the differences in cellular gene expression induced by HBV and HCV proteins. The expression of the majority of genes investigated differed only slightly between HBV and HCV protein-producing cells. However, HBV and HCV proteins clearly regulated several genes in a reciprocal manner. Combined, these microarray results shed new light on the effects of HBV proteins on cellular gene expression and on the differences in the pathogenic activities of these two hepatitis viruses. (C) 2002 Elsevier Science (USA). All rights reserved.