Ferroptosis is involved in PGPS-induced otitis media in C57BL/6 mice.
Ferroptosis is involved in PGPS-induced otitis media in C57BL/6 mice.
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铁死亡与 PGPS 诱导的 C57BL/6 小鼠中耳炎有关
DOI：
10.1038/s41420-022-01025-1
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发表时间：
2022-04-21
影响因子：
7
通讯作者：
Zheng Q
中科院分区：
文献类型：
--
作者：
Yan B;Xie D;Wu Y;Wang S;Zhang X;Zhao T;Liu L;Ma P;Li G;Yang Y;Zhao Y;Zheng T;Geng R;Li B;Zheng Q
Otitis media (OM) is a common disease that can cause hearing loss in children. Currently, the main clinical treatment for OM is antibiotics, but the overuse of antibiotics might lead to bacterial resistance, which is a worldwide public health challenge. Studying the pathogenesis of OM will help us develop new effective treatments. Ferroptosis is one type of programmed cell death characterized by the occurrence of lipid peroxidation driven by iron ions. Many studies have shown that ferroptosis is associated with infectious diseases. It is presently unclear whether ferroptosis is involved in the pathogenesis of OM. In this study, we explored the relationship between ferroptosis and OM by PGPS-induced OM in C57BL/6 mice and treating the induced OM with ferroptosis inhibitors deferoxamine (DFO), Ferrostatin-1 (Fer-1), and Liperoxstatin-1 (Lip-1). We examined the expression of ferroptosis-related proteins acyl-CoA synthetase long chain family member 4 (ACSL4) and prostaglandin-endoperoxide synthase 2 (Cox2), glutathione peroxidase 4 (GPX4) protein as well as lipid peroxidation markers 4-hydroxynonenal (4-HNE) and malondialdehyde (MDA). The results showed that in PGPS-induced OM model mice, several ferroptosis-related proteins including ACSL4 and Cox2 were up-regulated compared to mice treated with saline. Meanwhile, a ferroptosis-related protein GPX4 was down-regulated upon PGPS treatment. The DFO treatment in PGPS-inoculated mice effectively inhibited the development of OM. The inhibitors treatment caused a significant decrease in the expression of ACSL4, Cox2, 4 HNE, MDA, reduction in free iron. Meanwhile, the ferroptosis inhibitors treatment caused increase in the expression of inflammation-related factors tumor necrosis factor-α (TNF-α) and antioxidant protein GPX4. Our results suggest that there is a crosstalk between ferroptosis signaling pathway and the pathogenesis of OM. Ferroptosis inhibition can alleviate PGPS-induced OM.
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影响因子：
64.5
作者：
Dixon SJ;Lemberg KM;Lamprecht MR;Skouta R;Zaitsev EM;Gleason CE;Patel DN;Bauer AJ;Cantley AM;Yang WS;Morrison B 3rd;Stockwell BR
通讯作者：
Stockwell BR
DOI：
10.3390/healthcare9040458
发表时间：
2021-04-14
期刊：
Healthcare (Basel, Switzerland)
影响因子：
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作者：
Gavrilovici C;Spoială EL;Ivanov AV;Mocanu A;Ștreangă V;Alecsa MS;Miron I
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DOI：
10.1074/jbc.ra120.015779
发表时间：
2021-01
期刊：
The Journal of biological chemistry
影响因子：
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DOI：
10.1016/j.bbadis.2021.166261
发表时间：
2021-09-17
影响因子：
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作者：
Cui, Canqi;Li, Tingting;Gu, Yanyun
通讯作者：
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