Exploring the mechanism of Jieduquyuziyin prescription on systemic lupus erythematosus by GC-MS-based urine metabolomics
Exploring the mechanism of Jieduquyuziyin prescription on systemic lupus erythematosus by GC-MS-based urine metabolomics
复制标题
基于GC-MS的尿液代谢组学探讨解毒祛瘀滋饮方治疗系统性红斑狼疮的作用机制
DOI：
10.1002/bmc.4087
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发表时间：
2018
影响因子：
1.8
通讯作者：
Ding Xinghong
中科院分区：
文献类型：
--
作者：
Wei Jing;Gao Jun;Ding Xinghong
为了研究系统性红斑狼疮（SLE）的代谢特征和解毒祛瘀滋饮方的治疗效果，建立了基于气相色谱-质谱联用的尿液代谢组学方法。采用气相色谱-质谱法分析SLE模型小鼠(MRL/lpr)组、醋酸泼尼松治疗SLE小鼠组、解毒祛瘀滋饮方治疗SLE小鼠组和对照组(C57BL/6 J)给药后尿液标本的尿代谢特征。然后通过多变量分析（特别是 Mass Profiler Professional、SIMCA-P 和偏最小二乘判别分析）对这些代谢谱进行处理。根据偏最小二乘判别分析结果，SLE模型组与对照组明显分离，说明SLE的发病对代谢网络有较大影响，且SLE模型组与对照组在尿液代谢物方面存在显着性差异。鉴定出11个差异代谢物与SLE相关，差异代谢物鉴定结果显示，代谢物主要与能量代谢和氨基酸代谢途径相关。这些结果可为进一步探讨中药治疗SLE的作用机制提供实验依据。
A urine metabolomics method based on gas chromatography–mass spectrometry was developed in order to investigate the metabolite characteristics of systemic lupus erythematosus (SLE) and the therapeutic effects of Jieduquyuziyin prescription. The urinary metabolic profiles in urine specimens of the SLE model mice (MRL/lpr) group, prednisone acetate‐treated SLE mouse group, Jieduquyuziyin prescription‐treated SLE mouse group, and control group (C57BL/6 J) after the administration were analyzed by gas chromatography–mass spectrometry. These metabolic profiles were then processed by multivariate analysis, in particular Mass Profiler Professional, SIMCA‐P and partial least‐squares discriminant analysis. According to the partial least‐squares discriminant analysis results, the SLE model group and the control group were obviously separated, indicating that the incidence of SLE had a greater impact on the metabolic network, and the SLE model group had significant difference compared with the control group in urine metabolites. Eleven differential metabolites were identified to be related to SLE, and the results of differential metabolite identification showed that the metabolites were mainly related to energy metabolism and amino acid metabolism pathway. These results can provide an experimental basis for further exploring the mechanism of traditional Chinese medicine in the treatment of SLE.