Expression of the cancer stem cell markers CD44v6 and ABCG2 in tongue cancer: Effect of neoadjuvant chemotherapy on local recurrence
Expression of the cancer stem cell markers CD44v6 and ABCG2 in tongue cancer: Effect of neoadjuvant chemotherapy on local recurrence
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DOI：
10.3892/ijo.2014.2289
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发表时间：
2014-04-01
影响因子：
5.2
通讯作者：
Umeda, Masahiro
中科院分区：
文献类型：
--
作者：
Yanamoto, Souichi;Yamada, Shin-Ichi;Umeda, Masahiro
新辅助化疗（NAC）的疗效一直存在争议，目前尚无高证据水平支持NAC的报道。最近，我们发现，在许多NAC治疗的口腔鳞状细胞癌患者中，手术切缘很深，非常接近肿瘤部位，这表明NAC可能导致局部复发和不良结局。本研究的目的是利用肿瘤干细胞标记物免疫组织化学评价NAC对肿瘤局部复发的影响。我们回顾性分析了89例舌癌根治术患者，并检查NAC对肿瘤局部复发的影响。免疫组化检测肿瘤干细胞标志物CD 44 v6和ABCG 2的表达。在我们的研究中，局部复发率为12.4%。CD 44 v6和ABCG 2表达分别与区域淋巴结转移、浸润方式、浸润深度、神经周围浸润和局部复发显著相关。肿瘤局部复发是5年疾病特异性生存率的独立预测因素。NAC治疗患者中CD 44 v6或ABCG 2阳性与肿瘤局部复发显著相关。这表明NAC治疗病例的局部复发与癌症干细胞样细胞有关。我们认为NAC导致更具侵袭性的癌症干细胞样细胞的选择和/或残留。
The efficacy of neoadjuvant chemotherapy (NAC) is controversial, and no report supports NAC with a high evidence level. Recently, we showed that a deep surgical margin was resected very close to the tumor site in many NAC-treated oral squamous cell carcinoma patients, suggesting that NAC may lead to local recurrence and poor outcomes. The purpose of this study was to evaluate the effect of NAC on tumor local recurrence using cancer stem cell marker immunohistochemistry. We retrospectively analyzed 89 patients who underwent radical surgery for tongue cancer, and examined the effect of NAC on tumor local recurrence. Cancer stem cell marker (CD44v6 and ABCG2) expression was detected by immunohistochemistry. In our study, the local recurrence rate was 12.4%. CD44v6 and ABCG2 expression was significantly associated with regional lymph node metastasis, pattern of invasion, depth of invasion, perineural invasion and local recurrence, respectively. Tumor local recurrence was a significant independent predictive factor of the 5-year disease specific survival. CD44v6 or ABCG2 positivity in NAC-treated patients was significantly associated with tumor local recurrence. It was suggested that local recurrence in NAC-treated cases is associated with cancer stem-like cells. We propose that NAC leads to the selection and/or residue of more aggressive cancer stem-like cells.