Identification and bioactivity of the growth inhibitors in Tuber spp. methanolic extracts
Identification and bioactivity of the growth inhibitors in Tuber spp. methanolic extracts
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DOI：
10.1080/11263504.2014.983575
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发表时间：
2015-11-02
期刊：
影响因子：
2
通讯作者：
Venanzoni, R.
中科院分区：
文献类型：
--
作者：
Angelini, P.;Tirillini, B.;Venanzoni, R.
对块茎、黑孢子实体和博尔奇黑孢子实体的甲醇提取物的游离脂肪酸含量进行了测定，以确定其对块菌化感作用的贡献。亚油酸是所有松露品种中检测到的主要游离脂肪酸。块茎中除亚油酸外，还含有油酸和棕榈酸。萃取物。从总产量来看，黑孢霉菌和普通霉菌合成的所有已鉴定的游离脂肪酸的量显著高于马格纳霉菌和波氏霉菌。根据所鉴定化合物的比活性(EC50)，在拟南芥、莲花、纤毛Melica和Silene寻常草的生长上评价了它们的化感活性。亚油酸的比活力最低，棕榈酸的比活力最高。黑松露提取物比白松露提取物具有更高的抑制活性，这可以用它们中相对含量较高的FFA来解释。
The methanolic extracts of fresh fruiting bodies of Tuber melanosporum, T. aestivum, T. magnatum and T. borchii were evaluated for their free fatty acids (FFAs) content to test their contribution to truffle allelopathic activity. Linoleic acid was the major FFA detected in all truffle species. In addition to linoleic acid, oleic acid and palmitic acid were the other abundant FFAs in the Tuber spp. extracts. Looking at the total production, T. melanosporum and T. aestivum synthesized significantly higher amounts of all identified FFA than T. magnatum and T. borchii. The allelopathic activity, based on the specific activity (EC50) of the identified compounds, was evaluated in the growth of Arabidopsis thaliana, Lotus corniculatus, Melica ciliata and Silene vulgaris. Linoleic acid had the lowest specific activity and palmitic acid had the highest. The black truffle extracts had a higher inhibitory activity than white truffles, and this could be explained by the high relative amount of FFAs in them.