Neutralizing Antibodies Correlate with Protection from SARS-CoV-2 in Humans during a Fishery Vessel Outbreak with a High Attack Rate.
Neutralizing Antibodies Correlate with Protection from SARS-CoV-2 in Humans during a Fishery Vessel Outbreak with a High Attack Rate.
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DOI：
10.1128/jcm.02107-20
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发表时间：
2020-10-21
影响因子：
9.4
通讯作者：
Greninger AL
中科院分区：
文献类型：
--
作者：
Addetia A;Crawford KHD;Dingens A;Zhu H;Roychoudhury P;Huang ML;Jerome KR;Bloom JD;Greninger AL
通过鉴定人类保护的免疫学相关因素，将大大促进针对SARS-CoV-2的疫苗的开发。然而，迄今为止，保护性免疫的研究仅在动物模型中进行，尚未在人类中建立保护的相关性。在这里，我们描述了与高攻击率相关的渔船上的SARS-CoV-2的爆发。船上122人中有120人在出发前进行了血清学和病毒逆转录-聚合酶链反应（RT-PCR）检测沿着，并在返回海岸后进行了重复检测，平均随访时间为32年。通过鉴定人类保护的免疫学相关因素，将大大促进针对SARS-CoV-2的疫苗的开发。然而，迄今为止，保护性免疫的研究仅在动物模型中进行，尚未在人类中建立保护的相关性。在这里，我们描述了与高攻击率相关的渔船上的SARS-CoV-2的爆发。在32.5天（范围18.8至50.5天）的中位随访期内，对船上122人中的120人进行了出发前血清学和病毒逆转录-PCR（RT-PCR）检测沿着以及返回海岸后的重复检测。共有104人的RT-PCR病毒检测呈阳性，循环阈值（CT）<35或在随访期间发生血清转化，船上发病率为85.2%（104/122人）。对39个病毒基因组的宏基因组测序表明，此次疫情主要起源于一个单一的病毒分支。只有三名船员在该船出发前的初步血清学筛查中检测出血清阳性，在后续检测中也检测出中和抗体和加标反应抗体。在病毒爆发期间，没有一名具有中和抗体滴度的机组人员显示出真正的病毒感染证据或出现任何症状。因此，先前感染的中和抗体的存在与防止再感染显著相关（Fisher精确检验，P = 0.002）。
The development of vaccines against SARS-CoV-2 would be greatly facilitated by the identification of immunological correlates of protection in humans. However, to date, studies on protective immunity have been performed only in animal models and correlates of protection have not been established in humans. Here, we describe an outbreak of SARS-CoV-2 on a fishing vessel associated with a high attack rate. Predeparture serological and viral reverse transcription-PCR (RT-PCR) testing along with repeat testing after return to shore was available for 120 of the 122 persons on board over a median follow-up of 32. The development of vaccines against SARS-CoV-2 would be greatly facilitated by the identification of immunological correlates of protection in humans. However, to date, studies on protective immunity have been performed only in animal models and correlates of protection have not been established in humans. Here, we describe an outbreak of SARS-CoV-2 on a fishing vessel associated with a high attack rate. Predeparture serological and viral reverse transcription-PCR (RT-PCR) testing along with repeat testing after return to shore was available for 120 of the 122 persons on board over a median follow-up of 32.5 days (range, 18.8 to 50.5 days). A total of 104 individuals had an RT-PCR-positive viral test with a cycle threshold (CT) of <35 or seroconverted during the follow-up period, yielding an attack rate on board of 85.2% (104/122 individuals). Metagenomic sequencing of 39 viral genomes suggested that the outbreak originated largely from a single viral clade. Only three crew members tested seropositive prior to the boat’s departure in initial serological screening and also had neutralizing and spike-reactive antibodies in follow-up assays. None of the crew members with neutralizing antibody titers showed evidence of bona fide viral infection or experienced any symptoms during the viral outbreak. Therefore, the presence of neutralizing antibodies from prior infection was significantly associated with protection against reinfection (Fisher’s exact test, P = 0.002).