Comparison of performance and abnormal cell flagging of two automated hematology analyzers: Sysmex XN 3000 and Beckman Coulter DxH 800
Comparison of performance and abnormal cell flagging of two automated hematology analyzers: Sysmex XN 3000 and Beckman Coulter DxH 800
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DOI：
10.1111/ijlh.12717
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发表时间：
2017-12-01
影响因子：
3
通讯作者：
Omer, B.
中科院分区：
文献类型：
--
作者：
Genc, S.;Dervisoglu, E.;Omer, B.
简介：目的是评价Sysmex XN 3000和UniCel DxH 800的分析性能，将所得结果与手动计数进行比较，并相互比较。方法：对2142例全血样本进行分析评价。对因原始细胞、非典型淋巴细胞（AL）、未成熟粒细胞（IG）或有核红细胞（NRBC）而标记的样本进行显微镜检查（n=102）。XN 3000的血红蛋白、RBC、MCV、WBC、血小板低于5%，白细胞分类低于10%，单核细胞为15.6%。DxH 800的精密度结果也低于5.0%，血小板（9.5%）和单核细胞（45%）处于低水平。除单核细胞和嗜碱性粒细胞外，分析仪的比较显示出良好的一致性（R> 0.86）。XN 30 0 0对IGs、blasts和AL的标记灵敏度高于DxH 80 0，对NRBC的标记灵敏度相近。结论：XN 30 0 0和DxH 80 0是准确、高精密度的系统，可有效地用于大批量实验室。XN 3000在检测原始细胞、IGs和AL方面的标记灵敏度高于DxH 800。以高灵敏度检测异常细胞可以改善实验室工作流程，减少载玻片检查并加快周转时间。
Introduction: The purpose was to evaluate the analytical performances of Sysmex XN3000 and UniCel DxH 800 comparing the obtained results with manual counting and between each other. Also flagging capabilities of abnormal cells were compared for both analyzers.Methods: Two thousand one hundred and forty-two whole-blood samples were analyzed for evaluation. The samples flagged due to blast, atypical lymphocyte (AL), immature granulocyte (IG), or nucleated red blood cells (NRBC) were microscopically reviewed (n=102).Results: The within-run and between-day coefficient of variations (CV%) of XN 3000 for hemoglobin, RBC, MCV, WBC, and platelets were lower than 5% and for WBC differentials lower than 10% except monocytes which was 15.6% at low level. The precision results of DxH 800 were also lower than 5.0% except platelets (9.5%) and monocytes (45%) at low level. The comparison of analyzers revealed good agreement (R>.86), except monocytes and basophils. The flagging sensitivities of XN 3000 were higher for IGs, blasts, and ALs than those of DxH 800 and almost similar for NRBC.Conclusion: The XN 3000 and DxH 800 are accurate, highly precise systems and can be used effectively in high-volume laboratories. The flagging sensitivity of XN 3000 was higher in detecting blasts, IGs, and ALs than that of DxH 800. The detection of abnormal cells with high sensitivity may improve laboratory workflow with a reduced slide review and accelerated turnaround time.