NKG2A complexed with CD94 defines a novel inhibitory natural killer cell receptor.
NKG2A complexed with CD94 defines a novel inhibitory natural killer cell receptor.
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NKG2A 与 CD94 复合定义了一种新型抑制性自然杀伤细胞受体。
DOI：
10.1084/jem.185.4.795
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发表时间：
1997-02-17
期刊：
影响因子：
--
通讯作者：
Coligan JE
中科院分区：
文献类型：
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作者：
Brooks AG;Posch PE;Scorzelli CJ;Borrego F;Coligan JE
CD 94是由自然杀伤（NK）细胞和T细胞亚群表达的C型凝集素。使用抗CD 94单克隆抗体的阻断研究表明，它是人类白细胞抗原I类分子的受体。CD 94最近被证明是一种26-kD蛋白，与一种未鉴定的43-kD蛋白共价结合。本报告显示，NKG 2A是一种43 kD蛋白，与NK细胞表面的CD 94共价结合。NKG 2A的细胞表面表达依赖于与CD 94的结合，因为成熟蛋白的特征性糖基化模式仅在与CD 94相关的NKG 2A中发现。NK细胞克隆的分析表明，NKG 2A在所有NK细胞克隆中表达，其CD 16依赖性杀伤被交联CD 94抑制。抑制信号的诱导与NKG 2A胞质结构域上存在两个基于免疫受体酪氨酸的抑制基序（V/LXYXXL）一致。在Ly 49和杀伤细胞抑制性受体上发现了类似的基序，它们也向NK细胞传递负信号。
CD94 is a C-type lectin expressed by natural killer (NK) cells and a subset of T cells. Blocking studies using anti-CD94 mAbs have suggested that it is a receptor for human leukocyte antigen class I molecules. CD94 has recently been shown to be a 26-kD protein covalently associated with an unidentified 43-kD protein(s). This report shows that NKG2A, a 43-kD protein, is covalently associated with CD94 on the surface of NK cells. Cell surface expression of NKG2A is dependent on the association with CD94 as glycosylation patterns characteristic of mature proteins are found only in NKG2A that is associated with CD94. Analysis of NK cell clones showed that NKG2A was expressed in all NK cell clones whose CD16-dependent killing was inhibited by cross-linking CD94. The induction of an inhibitory signal is consistent with the presence of two immunoreceptor tyrosine-based inhibitory motifs (V/LXYXXL) on the cytoplasmic domain of NKG2A. Similar motifs are found on Ly49 and killer cell inhibitory receptors, which also transmit negative signals to NK cells.