The trophoblast survival capacity in preeclampsia
The trophoblast survival capacity in preeclampsia
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DOI：
10.1371/journal.pone.0186909
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发表时间：
2017-11-06
期刊：
影响因子：
3.7
通讯作者：
Huppertz, Berthold
中科院分区：
文献类型：
--
作者：
Hutabarat, Martina;Wibowo, Noroyono;Huppertz, Berthold
背景子痫前期已经成为世界上主要的孕产妇健康问题，给母亲、新生儿以及卫生系统带来了巨大的负担。先兆子痫的发病机制似乎包括妊娠早期影响胎盘绒毛滋养细胞分化的事件。所产生的变化的细胞死亡谱从细胞凋亡，通过增加自噬和坏死坏死，坏死反过来诱导全身炎症的mother. MethodsPlacentaltissue样品和母体血清样品从40名孕妇收集正常妊娠，IUGR，早发性和晚发性先兆子痫。使用系统随机取样技术定量LC 3B和Beclin-1的免疫组织化学。LDH和其他标志物的血清水平进行了评估serum.ResultsExpression的自噬标志物LC 3B和Beclin-1是组间显着不同的LC 3B/Beclin-1的比例。早发型先兆子痫和IUGR的自噬蛋白表达水平最高，而正常妊娠和晚发型先兆子痫的LC 3B/Beclin-1比值最高。早发型子痫前期与细胞缺陷标志物游离LDH呈最高的负相关性。结论自噬在绒毛滋养层细胞死亡谱和细胞存活能力中起关键作用。自噬蛋白表达的改变与病理性妊娠如先兆子痫有关。
BackgroundPreeclampsia has become the world's major maternal health problem putting a huge burden on mothers, newborns and also on the health systems. The pathogenesis of preeclampsia seems to include events in very early pregnancy affecting differentiation of placental villous trophoblast. The arising changes of the cell death spectrum from apoptosis via increased autophagy and aponecrosis to necrosis in turn induce systemic inflammation of the mother.MethodsPlacental tissue samples and maternal serum samples from 40 pregnant women were collected from normal pregnancy, IUGR, early-onset and late-onset preeclampsia. Immunohistochemistry for LC3B and Beclin-1 was quantified using systematic random sampling techniques. Serum levels of LDH and other markers were assessed in serum.ResultsExpression of the autophagy markers LC3B and Beclin-1 was significantly different between groups as was the LC3B/Beclin-1 ratio. Early-onset preeclampsia and IUGR had the highest autophagy protein expression levels, while normal pregnancy and late-onset preeclampsia had the highest LC3B/Beclin-1 ratio. Early-onset preeclampsia had the highest negative correlation with free LDH as cell defect marker.ConclusionsAutophagy plays a critical role in the cell death spectrum and cellular survival capacity of villous trophoblast. Alterations in autophagic protein expression are involved in pathological pregnancies such as preeclampsia.