Voltage-gated proton "channels": a spectator's viewpoint.
Voltage-gated proton "channels": a spectator's viewpoint.
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DOI：
10.1085/jgp.20028706
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发表时间：
2002-12
期刊：
影响因子：
--
通讯作者：
Grinstein S
中科院分区：
文献类型：
--
作者：
Touret N;Grinstein S
在激活和/或去极化的吞噬细胞中记录的电压门控、H+选择电导的分子特性正在引起激烈的争论。在走出这个舞台后，我们感到不支持任何特定的观点，并希望能够在下面就我们认为已经明确确立的内容以及在发表结论性声明之前需要进一步确认的内容提供不偏不倚的意见。先天免疫系统是抵御微生物入侵的第一道防线。这种免疫反应是由特殊的细胞，巨噬细胞、中性粒细胞、嗜酸性粒细胞和树突状细胞来完成的，它们识别、摄取并摧毁病原生物。这些专业的吞噬细胞已经开发出了一系列杀灭微生物的策略，旨在清除生物体中潜在的感染因子。在被吞噬后，外来生物被困在一个叫做吞噬体的特殊囊泡中，通过一系列与其他膜室协调的裂变和融合事件，吞噬体获得了杀死微生物的能力，最终形成了吞噬体。这些成熟事件包括液泡质子泵和其他整合蛋白进入吞噬体膜，以及将杀微生物酶和裂解酶输送到液泡腔。
A battle is raging over the molecular identity of the voltage-gated, H+-selective conductance that has been recorded in activated and/or depolarized phagocytes. Having stepped out of this arena, we feel uncommitted to any particular viewpoint and hope to be able to provide below an unbiased opinion of what we feel has been established unambiguously, and what requires additional confirmation before conclusive statements can be issued.The innate immune system is the first line of defense against invading microorganisms. This immune response is performed by specialized cells, macrophages, neutrophils, eosinophils, and dendritic cells, that recognize, ingest, and destroy pathogenic organisms. These professional phagocytes have developed an arsenal of microbicidal strategies aimed at ridding the organism of potential infectious agents. After engulfment, the foreign organism is trapped in a specialized vesicle, called the phagosome, which acquires microbicidal ability as a result of a series of coordinated fission and fusion events with other endomembrane compartments, culminating in the formation of the phagolysosome. These maturation events include the incorporation of vacuolar proton pumps and other integral proteins into the phagosomal membrane, as well as the delivery of microbicidal and lytic enzymes into the vacuolar lumen.