Determination of underivatized amino acid δ13C by liquid chromatography/isotope ratio mass spectrometry for nutritional studies:: the effect of dietary non-essential amino acid profile on the isotopic signature of individual amino acids in fish
Determination of underivatized amino acid δ13C by liquid chromatography/isotope ratio mass spectrometry for nutritional studies:: the effect of dietary non-essential amino acid profile on the isotopic signature of individual amino acids in fish
复制标题
DOI：
10.1002/rcm.3554
复制
发表时间：
2008-06-01
影响因子：
2
通讯作者：
Focken, Ulfert
中科院分区：
文献类型：
--
作者：
McCullagh, James;Gaye-Siessegger, Julia;Focken, Ulfert
本研究利用液相色谱-同位素比值质谱联用技术（LCARMS）研究了虹鳟鱼（Oncorhynchus mykiss）饲料中非必需氨基酸组成对个体氨基酸δ C-13值的影响。在这个实验中，鳟鱼饲养控制饮食或三个实验饮食，不同的非必需/条件必需氨基酸的组成，为期6周。对照饮食是以鱼粉作为主要蛋白质来源的市售鳟鱼起始饲料。实验饮食不含蛋白质，只有合成氨基酸。饮食1在必需和非必需氨基酸方面与鱼粉的组成相似，饮食2具有所有必需氨基酸，但半胱氨酸、甘氨酸、脯氨酸和酪氨酸被相应量的其前体取代，并且在饮食3中，所有非必需氨基酸被谷氨酸取代。LC/IRMS用于测定饲料和组织中单个氨基酸的Δ C-13值，无需衍生化。饮食影响鱼中单个氨基酸的Δ C-13。对于饮食1-3的鱼，氨基酸Δ C-13值显示出类似的趋势：苯丙氨酸从饮食到身体组织显示出非常小的变化。精氨酸，赖氨酸，酪氨酸和脯氨酸表现出强烈的消耗从饮食到身体组织和甘氨酸，丙氨酸，天冬氨酸和丝氨酸都表现出可变的，但强烈的C-13富集。在确定所有氨基酸δ C-13值之前，改进是必要的;然而，这项研究表明，通过LC/IRMS测量氨基酸同位素特征对营养生理学家来说是一种很有前途的新技术。版权所有（C）2008约翰威利父子有限公司
This study provides data for the effect of dietary non-essential amino acid composition on the delta C-13 values of individual amino acids in rainbow trout (Oncorhynchus mykiss) using liquid chromatography coupled to isotope ratio mass spectrometry (LCARMS). In this experiment, trout were reared either on a control diet or on three experimental diets, differing in the composition of non-essential/conditionally essential amino acids, for a period of 6 weeks. The control diet was, a commercial trout starter feed with fish meal as the main protein source. The experimental diets contained no protein, only synthetic amino acids. Diet 1 resembled the composition of fish meal in both essential and non-essential amino acids, Diet 2 had all essential amino acids, but cysteine, glycine, proline and tyrosine were replaced by the corresponding amounts of their precursors, and in Diet 3 all non-essential amino acids were replaced by glutamate. LC/IRMS was used for the determination of delta C-13 values of individual amino acids from diets and tissues without derivatization. Diet affected the delta C-13 of individual amino acids in fish. For fish on Diets 1-3 amino acid delta C-13 values showed a similar trend: phenylalanine showed very little change from diet to body tissue. Arginine, lysine, tyrosine and proline showed strong depletion from diet to body tissue and glycine, alanine, aspartate and serine all showed variable but strong enrichment in C-13. Improvements are necessary before all amino acid delta C-13 values can be determined; however, this study demonstrates that measuring amino acid isotopic signatures by LC/IRMS is a promising new technique for nutritional physiologists. Copyright (C) 2008 John Wiley & Sons, Ltd.