Electromagnetic stirring in a microbioreactor with non-conventional chamber morphology and implementation of multiplexed mixing.
Electromagnetic stirring in a microbioreactor with non-conventional chamber morphology and implementation of multiplexed mixing.
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DOI：
10.1002/jctb.4762
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发表时间：
2015-10
期刊：
影响因子：
--
通讯作者：
Szita N
中科院分区：
文献类型：
--
作者：
Tan CK;Davies MJ;McCluskey DK;Munro IR;Nweke MC;Tracey MC;Szita N
微生物反应器已经成为早期生物过程开发的新工具。混合是生物反应器运行的核心(在所有范围内)。因此，微搅拌方法的成功实施对微生物反应器技术的进一步发展至关重要。这项研究的目的是开发一种微搅拌方法，以帮助稳健的微生物反应器操作，并促进经济有效的并行化。利用一种新颖的微搅拌方法开发了一种微生物反应器，该方法通过顺序地激活一环电磁铁来移动磁珠。微搅拌法在反应室设计中提供了灵活性，混合在圆柱形、菱形和三角形的反应室中得到了演示。对不同的电磁铁开/关顺序进行了混合分析，圆柱形、菱形和三角形燃烧室的混合时间分别为4.5 S、2.9 S和2.5 S。无微泡引爆的简易性是圆柱形微生物反应器的一个典型挑战，它是通过钻石形室获得的。在双组分系统中，各组成反应器之间的混合行为是一致的。介绍了一种利用电磁驱动的新型搅拌方法，该方法搅拌速度快，易于与微生物反应器集成。获得的设计灵活性使制造适合微流控操作的腔室成为可能，双工演示器突出了具有成本效益的并行化潜力。结合以前发表的盒式微生物反应器的制造，这些进展将促进稳健和并行的微生物反应器的发展。©2015作者。《化学技术与生物技术》杂志由John Wiley&Sons Ltd代表化学工业学会出版。
Microbioreactors have emerged as novel tools for early bioprocess development. Mixing lies at the heart of bioreactor operation (at all scales). The successful implementation of micro‐stirring methods is thus central to the further advancement of microbioreactor technology. The aim of this study was to develop a micro‐stirring method that aids robust microbioreactor operation and facilitates cost‐effective parallelization. A microbioreactor was developed with a novel micro‐stirring method involving the movement of a magnetic bead by sequenced activation of a ring of electromagnets. The micro‐stirring method offers flexibility in chamber designs, and mixing is demonstrated in cylindrical, diamond and triangular shaped reactor chambers. Mixing was analyzed for different electromagnet on/off sequences; mixing times of 4.5 s, 2.9 s, and 2.5 s were achieved for cylindrical, diamond and triangular shaped chambers, respectively. Ease of micro‐bubble free priming, a typical challenge of cylindrical shaped microbioreactor chambers, was obtained with a diamond‐shaped chamber. Consistent mixing behavior was observed between the constituent reactors in a duplex system. A novel stirring method using electromagnetic actuation offering rapid mixing and easy integration with microbioreactors was characterized. The design flexibility gained enables fabrication of chambers suitable for microfluidic operation, and a duplex demonstrator highlights potential for cost‐effective parallelization. Combined with a previously published cassette‐like fabrication of microbioreactors, these advances will facilitate the development of robust and parallelized microbioreactors. © 2015 The Authors. Journal of Chemical Technology & Biotechnology published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.