Decay rates of human mRNAs: Correlation with functional characteristics and sequence attributes
Decay rates of human mRNAs: Correlation with functional characteristics and sequence attributes
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DOI：
10.1101/gr.1272403
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发表时间：
2003-08-01
期刊：
影响因子：
7
通讯作者：
Darnell, JE
中科院分区：
文献类型：
--
作者：
Yang, E;van Nimwegen, E;Darnell, JE
尽管mRNA衰减率是任何给定mRNA物种稳态浓度的关键决定因素，但在种群水平上，关于影响周转率的因素以及这些速率如何融入细胞决策的了解相对较少。我们决定用高密度寡核苷酸阵列测量两种人类细胞系的mRNA衰减率，这种阵列可以同时测量数千种mRNA的衰减率。利用现有的注释和生物过程的基因本体层次结构，我们将mrna分配到不同分辨率的功能类别，并比较这些类别之间的衰减率统计数据。结果显示，在功能类之间衰减率的变化中，具有统计意义的组织原则。特别是，与其他转录物相比，转录因子mrna的平均衰变率增加，并且富含具有半衰期的“快速衰变”mrna
Although mRNA decay rates are a key determinant of the steady-state concentration for any given mRNA species, relatively little is known, on a Population level, about what factors influence turnover rates and how these rates are integrated into cellular decisions. We decided to measure mRNA decay rates in two human cell lines with high-density oligonucleotide arrays that enable the measurement of decay rates simultaneously for thousands of mRNA species. Using existing annotation and the Gene Ontology hierarchy of biological processes, we assign mRNAs to functional classes at various levels of resolution and compare the decay rate statistics between these classes. The results show statistically significant organizational principles in the variation of decay rates among functional classes. In particular, transcription factor mRNAs have increased average decay rates compared with other transcripts and are enriched in "fast-decaying" mRNAs with half-lives