Hepatic metabolism by31P NMR
Hepatic metabolism by31P NMR
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31 P NMR 测定肝脏代谢
作者：
R. Iles;J. Griffiths
用~（31）P核磁共振（NMR）波谱法研究了完整肝脏的肝脏代谢。正常肝脏的31 P NMR光谱检测到很少的ADP和比酶或化学分析发现的低得多的无机磷酸盐。持续30分钟的缺血引起ATP迅速下降到检测不到的水平;再流使ATP浓度恢复到其先前水平的70%。细胞内pH值的变化是定性相似的。果糖和甘油都引起ATP和无机磷酸盐的快速福尔斯下降;在果糖的情况下，这些原始浓度在30分钟后几乎恢复，但在甘油的情况下，它们仍然被抑制。
Hepatic metabolism in intact livers has been studied by 31P nuclear magnetic resonance (NMR) spectroscopy. 31P NMR spectroscopy of normal liver detects little ADP and much lower amounts of inorganic phosphate than are found by enzymatic or chemical analysis. Ischaemia of 30 min duration provokes a rapid fall in ATP to undetectable levels; reflow restores the ATP concentration to 70% of its former level. Intracellular pH changes are qualitatively similar. Fructose and glycerol both cause rapid falls in ATP and inorganic phosphate; these original concentrations are almost restored after 30 min in the case of fructose but not with glycerol where they remain depressed.