Properties of a cytopathic agent isolated from a patient with subacute sclerosing panencephalitis in Japan.
Properties of a cytopathic agent isolated from a patient with subacute sclerosing panencephalitis in Japan.
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从日本亚急性硬化性全脑炎患者中分离出的细胞病变剂的特性。
DOI：
10.7883/yoken1952.25.321
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发表时间：
1972
期刊：
影响因子：
--
通讯作者：
Tadao Tsubaki
中科院分区：
文献类型：
--
作者：
Yutaka Doi;T. Sanpe;Minoru Nakajima;Shoichiro Okawa;Toshitada Katoh;Heihachi Itoh;Takeshi Sato;Kimio Oguchi;Toshiro Kumanishi;Tadao Tsubaki
从亚急性硬化性全脑炎（SSPE）患者的脑活检中分离出一种细胞病变因子。该代理可以最容易地保持感染的细胞与新鲜的Vero细胞（猕猴肾线）的共培养。细胞致病性包括在液体覆盖下单层上出现肉眼可见的圆形合胞体（斑块）。到目前为止，直到第60代水平，在各种条件下都没有获得无细胞病毒。麻疹病毒的抗原性通过免疫荧光试验和使用特异性麻疹病毒抗血清的空斑减少试验来证明。此外，副粘病毒的核衣壳在感染细胞，红细胞吸附的猕猴红细胞和繁殖特性的放线菌素D和5-溴脱氧尿苷的存在下，电子显微镜下的结果是一致的麻疹病毒。核衣壳在细胞核中含量丰富，而在细胞质中含量较少，这与其他人报道的SSPE病毒的数据相一致。成年豚鼠、小鼠和仓鼠通过脑内接种该制剂出现神经系统症状，大多数动物随后死亡。在-70 ℃下用二甲基亚砜冷冻储存能够将感染的细胞与未感染的Vero细胞一样长时间保存。这可能表明该试剂与宿主细胞的温和结合以及无细胞病毒的不可恢复性。
A cytopathic agent isolated from the brain biopsy of a patient with subacute sclerosing panencephalitis (SSPE) was investigated. The agent could most easily be maintained by cocultivation of infected cells with fresh Vero cells (cercopithecus monkey kidney line). Cytopathogenicity consisting of macroscopically recognizable round syncytia (plaques) appeared on the monolayer under liquid overlay. So far up to the 60th passage level, no cell-free virus has been obtained under various conditions. Antigenicity of measles virus was demonstrated by the immunofluorescence tests and by the plaque reduction tests using specific measles virus antiserum. In addition, electron microscopic findings of paramyxovirus nucleocapsids in the infected cells, hemadsorption of cercopithecus monkey erythrocytes and propagation characteristics in the presence of actinomycin D and of 5-bromodeoxyuridine were consistent with those of measles virus. That the nucleocapsids were abundant in the nuclei and much fewer in the cytoplasm is compatible with the data on SSPE viruses reported by others. Adult guinea pigs, mice and hamsters developed neurological symptoms by intracerebral inoculations of the agent and deaths ensued in most of the animals. Freezing storage with dimethyl sulfoxide at -70C was able to preserve the infected cells for a long period just as with uninfected Vero cells. This may suggest a temperate association of the agent with host cells and nonrecovery of cell-free virus.