SmJAZ8 acts as a core repressor regulating JA-induced biosynthesis of salvianolic acids and tanshinones in Salvia miltiorrhiza hairy roots
SmJAZ8 acts as a core repressor regulating JA-induced biosynthesis of salvianolic acids and tanshinones in Salvia miltiorrhiza hairy roots
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SmJAZ8 作为核心阻遏蛋白，调节丹参毛状根中 JA 诱导的丹酚酸和丹参酮的生物合成
DOI：
10.1093/jxb/erx484
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发表时间：
2018-03-16
影响因子：
6.9
通讯作者：
Liang, Zongsuo
中科院分区：
文献类型：
--
作者：
Pei, Tianlin;Ma, Pengda;Liang, Zongsuo
茉莉酸是一种重要的植物激素，调节植物的生长发育和防御过程，包括次生代谢产物的合成。JAZ蛋白是植物茉莉酸信号传导途径中的负调节因子。首次证实茉莉酸甲酯（MeJA）通过诱导丹参毛状根中丹参酮和丹参酸生物合成途径基因的表达，促进丹参毛状根中丹参酮和丹参酸的积累。从丹参中克隆了9个JAZ基因，它们在丹参毛状根中的表达量均被MeJA处理提高。然而，当详细分析时，SmJAZ 8在诱导的毛状根中显示出最强的表达。SmJAZ 8的过表达或RNAi分别下调或上调了MeJA诱导的转基因毛状根中丹参素酸和丹参酮的产量，并且转录因子和生物合成途径基因显示出反映化合物生产的表达模式。SmJAZ 8的遗传转化改变了其他SmJAZ基因的表达，这表明JAZ调节的次级代谢中发生串扰的证据。此外，转录组分析揭示了SmJAZ 8调节的初级-次级代谢平衡。总之，我们提出了一个新的作用SmJAZ 8作为一个负反馈回路控制器在JA诱导的丹参酸和丹参酮的生物合成。
Jasmonates (JAs) are important plant hormones that regulate a variety of plant development and defense processes, including biosynthesis of secondary metabolites. The JASMONATE ZIM DOMAIN (JAZ) proteins act as negative regulators in the JA signaling pathways of plants. We first verified that methyl jasmonate (MeJA) enhanced the accumulation of both salvianolic acids and tanshinones in Salvia miltiorrhiza (Danshen) hairy roots by inducing the expression of their biosynthetic pathway genes. Nine JAZ genes were cloned from Danshen and their expression levels in hairy roots were all increased by treatment with MeJA. When analyzed in detail, however, SmJAZ8 showed the strongest expression in the induced hairy roots. Overexpression or RNAi of SmJAZ8 deregulated or up-regulated the yields of salvianolic acids and tanshinones in the MeJA-induced transgenic hairy roots, respectively, and transcription factors and biosynthetic pathway genes showed an expression pattern that mirrored the production of the compounds. Genetic transformation of SmJAZ8 altered the expression of other SmJAZ genes, suggesting evidence of crosstalk occurring in JAZ-regulated secondary metabolism. Furthermore, the transcriptome analysis revealed a primary-secondary metabolism balance regulated by SmJAZ8. Altogether, we propose a novel role for SmJAZ8 as a negative feedback loop controller in the JA-induced biosynthesis of salvianolic acids and tanshinones.