Microtubule protein ADP-ribosylation in vitro leads to assembly inhibition and rapid depolymerization.
Microtubule protein ADP-ribosylation in vitro leads to assembly inhibition and rapid depolymerization.
复制标题
体外微管蛋白 ADP 核糖基化会导致组装抑制和快速解聚。
DOI：
10.1021/bi00116a042
复制
发表时间：
1992
期刊：
影响因子：
2.9
通讯作者：
Purich,DL
中科院分区：
文献类型：
--
作者：
Scaife,RM;Wilson,L;Purich,DL
材料和方法牛脑微管蛋白和HMW-MAP的分离、凝胶电泳和印迹。通过体外组装和拆卸循环获得牛脑微管蛋白（Margolis等人，1986年）。微管蛋白和HMW-MAP通过磷酸纤维素色谱法在MME缓冲液[100 mM 2-（N-吗啉代）乙磺酸，pH 6.8，
Materials and Methods Isolation of Bovine Brain Tubulin and HMW-MAPs, Gel Electrophoresis, and Blotting. Bovine brain microtubule protein was obtained by cycles of assembly and disassembly in vitro (Margolis et al., 1986). Tubulin and the HMW-MAPs were separated by phosphocellulose chromatography in MME buffer [100 mM 2-(7V-morpholino) ethanesulfonic acid, pH 6.8,