ACTIVATION OF TRYPANOSOME SURFACE GLYCOPROTEIN GENES INVOLVES A DUPLICATION-TRANSPOSITION LEADING TO AN ALTERED 3' END
ACTIVATION OF TRYPANOSOME SURFACE GLYCOPROTEIN GENES INVOLVES A DUPLICATION-TRANSPOSITION LEADING TO AN ALTERED 3' END
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DOI：
10.1016/0092-8674(81)90391-3
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发表时间：
1981-01-01
期刊：
影响因子：
64.5
通讯作者：
CROSS, GAM
中科院分区：
文献类型：
--
作者：
BERNARDS, A;VANDERPLOEG, LHT;CROSS, GAM
在T.布氏杆菌伴随着这些基因的额外拷贝的出现，即表达连锁拷贝，其在周围的限制性酶切位点上与相应的基因基本拷贝不同。表达连锁拷贝显然是用于mRNA合成的拷贝。克隆的基本拷贝基因含有相应mRNA的核苷酸序列，除了poly（A）尾之前的最后100-150个核苷酸。117个基本拷贝基因和117个互补DNA的3“-末端序列的比较表明，该区域的差异在于多个点突变、插入和缺失，差异始于编码序列。118互补DNA的3“-末端部分中的Bsp I位点存在于表达连锁拷贝中，但不存在于基本拷贝基因中。表达连锁拷贝是活性基因，并且表达连锁拷贝的产生涉及重复转座，其中基因的3“末端被替换。
Expression of the genes for variant surface glycoproteins 117 and 118 in T. brucei is accompanied by the appearance of an extra copy of these genes, the expression-linked copy, which differs in the surrounding restriction enzyme sites from the corresponding basic copy of the genes. The expression-linked copy is evidently the one used for mRNA synthesis. Cloned basic-copy genes contain the nucleotide sequence of the corresponding mRNA except for the last 100-150 nucleotides before the poly(A) tail. Comparison of the 3''-terminal sequence of the 117 basic-copy gene and the 117 complementary DNA shows this region differs by multiple point mutations, insertions and deletions, the differences starting within the coding sequence. A Bsp I site in the 3''-terminal part of the 118 complementary DNA is present in the expression-linked copy but not in the basic-copy gene. Expression-linked copies are the active genes, and that the generation of expression-linked copies involves a duplication-transposition in which the 3'' end of the gene is replaced.