Independent ingestion and microstructure of feeding patterns in infant rats lacking CCK-1 receptors
Independent ingestion and microstructure of feeding patterns in infant rats lacking CCK-1 receptors
复制标题
DOI：
10.1152/ajpregu.00379.2005
复制
发表时间：
2006-01-01
影响因子：
2.8
通讯作者：
Weller, A
中科院分区：
文献类型：
--
作者：
Blumberg, S;Haba, D;Weller, A
大冢Long-Evans Tokushima fatty （OLETF）大鼠是一种不表达CCK-1受体的Long-Evans Tokushima Otsuka （LETO）大鼠，发生于成年期、贪食、肥胖和非胰岛素依赖型糖尿病（NIDDM）。在OLETF和LETO幼鼠出生后第2-4天和第9-11天进行的30分钟独立进食试验中，我们检查了它们的体重增加和进食模式。在两个年龄段，OLETF的幼崽都明显比LETO对照组重，而且它们摄入的甜奶明显更多。摄入量的差异可归因于餐量和持续时间的显著增加。OLETF大鼠幼崽在一餐中舔的簇和簇的数量更多，在簇的大小和簇的大小上没有差异。OLETF和LETO幼崽的舔食间隔（ILI）无显著差异。这一指标在9-11天较2-4天降低。OLETF与LETO幼崽在第2-4天开始进食的潜伏期明显较短，但这种差异在第二次测试中消失。2 - 4天大的OLETF幼崽在喂食的第一分钟内消耗了更多的牛奶，与LETO对照组相比，它们的初始舔舐率和舔舐率衰减明显更大。因此，缺乏CCK-1受体或其他与oletf相关的异常，会导致饱腹感不足，导致餐量增加、贪食和体重增加，早在出生后2-4天。
Otsuka Long-Evans Tokushima fatty ( OLETF) rats are a strain of Long-Evans Tokushima Otsuka (LETO) rats that do not express CCK-1 receptors, developing in adulthood, hyperphagia, obesity, and non-insulin-dependent diabetes mellitus ( NIDDM). We examined weight gain and meal patterns during a 30-min independent ingestion test on postnatal days 2-4 and again on days 9-11 in OLETF and LETO rat pups. OLETF pups were significantly heavier compared with their LETO controls at both ages, and they consumed significantly more of the sweet milk diet. The difference in intake can be attributed to a significant increase in meal size and duration. Number of clusters and bursts of licking within a meal were greater in OLETF rat pups, with no difference between strains in burst and cluster size. Interlick interval (ILI) was not significantly different between OLETF and LETO pups. This measure decreased on days 9-11 compared with days 2-4 in both strains. Latency to start feeding was significantly shorter on days 2-4 in OLETF vs. LETO pups, but this difference disappeared at the second test at the older age. Two-to four-day-old OLETF pups consumed a larger volume of milk during the first minute of feeding, and their initial lick rate and decay of lick rate were significantly larger compared with their LETO controls. Lack of CCK-1 receptors, or other OLETF-related abnormalities, therefore, resulted in a satiation deficit, leading to increased meal size, hyperphagia, and increased weight gain as early as 2-4 postnatal days.