Integrating miRNA and mRNA Expression Profiling Uncovers miRNAs Underlying Fat Deposition in Sheep.
Integrating miRNA and mRNA Expression Profiling Uncovers miRNAs Underlying Fat Deposition in Sheep.
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整合 miRNA 和 mRNA 表达谱揭示绵羊脂肪沉积背后的 miRNA
DOI：
10.1155/2017/1857580
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发表时间：
2017
影响因子：
--
通讯作者：
Chen Y
中科院分区：
文献类型：
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作者：
Zhou G;Wang X;Yuan C;Kang D;Xu X;Zhou J;Geng R;Yang Y;Yang Z;Chen Y
microRNA（miRNAs）是一种内源性非编码RNA，可调控脂肪形成和脂肪代谢等多种生物学过程。在这里，我们采用了深度测序方法来确定脂肪组织中脂肪沉积相关miRNA的身份和丰度，这些脂肪组织来自肥尾（哈萨克斯坦绵羊，KS）和细尾（西藏绵羊，TS）绵羊品种。通过比较这两个品种的HiSeq数据，539个miRNA在两个品种中共有，而179个和97个miRNA分别在KS和TS中独特表达。我们还鉴定了35种被认为是推定的新型miRNAs。miRNA-mRNA的整合分析表明，miRNA-mRNA相关靶点主要参与细胞和代谢过程的基因本体（GO）生物学过程，miRNAs通过调节脂肪沉积的基本途径发挥重要作用。这些通路包括MAPK信号通路、FoxO和Wnt信号通路以及粘着斑。总之，我们的研究结果定义了可能有助于绵羊脂肪沉积和脂质代谢的miRNA表达特征。
MicroRNAs (miRNAs) are endogenous, noncoding RNAs that regulate various biological processes including adipogenesis and fat metabolism. Here, we adopted a deep sequencing approach to determine the identity and abundance of miRNAs involved in fat deposition in adipose tissues from fat-tailed (Kazakhstan sheep, KS) and thin-tailed (Tibetan sheep, TS) sheep breeds. By comparing HiSeq data of these two breeds, 539 miRNAs were shared in both breeds, whereas 179 and 97 miRNAs were uniquely expressed in KS and TS, respectively. We also identified 35 miRNAs that are considered to be putative novel miRNAs. The integration of miRNA-mRNA analysis revealed that miRNA-associated targets were mainly involved in the gene ontology (GO) biological processes concerning cellular process and metabolic process, and miRNAs play critical roles in fat deposition through their ability to regulate fundamental pathways. These pathways included the MAPK signaling pathway, FoxO and Wnt signaling pathway, and focal adhesion. Taken together, our results define miRNA expression signatures that may contribute to fat deposition and lipid metabolism in sheep.