Enhancing Menaquinone-7 Production by Bacillus natto R127 Through the Nutritional Factors and Surfactant
Enhancing Menaquinone-7 Production by Bacillus natto R127 Through the Nutritional Factors and Surfactant
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通过营养因子和表面活性剂提高纳豆芽孢杆菌 R127 的 Menaquinone-7 产量
DOI：
10.1007/s12010-017-2423-6
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发表时间：
2017-08-01
影响因子：
3
通讯作者：
Huang, He
中科院分区：
文献类型：
--
作者：
Hu, Xue-chao;Liu, Wei-ming;Huang, He
纳豆芽孢杆菌通常用于甲基萘醌-7的工业生产，甲基萘醌-7是一种重要的维生素，对血液凝固起着至关重要的作用，可能有助于预防心血管疾病和骨质疏松症。本研究确定了关键营养素的最佳组合，并建立了在耦合介质中有效使用表面活性剂以提高胞外MK-7的产量。在甘油53.6 g/L、大豆蛋白胨100 g/L、磷酸氢二钾10 g/L的最佳条件下，MK-7的产率为31.18 mg/L。在对数生长期添加20 g/L大豆油时，MK-7的最大产量为40.96 mg/L，分泌率为61.1%。非离子表面活性剂Span 20在提高产率方面是第二有希望的表面活性剂，而添加2g/L甜菜碱对MK-7的分泌率的影响最小，为19.1%。结果表明，表面活性剂的补充是调节细胞膜通透性的有效策略。图形摘要
Bacillus natto is commonly used in industrial production of menaquinone-7, an important vitamin which plays a crucial role for blood clotting and may contribute to prevention of cardiovascular disease and osteoporosis. This study determined the optimal combination of key nutrients and established an effective use of surfactant in a coupling medium to enhance the yield of extracellular MK-7. MK-7 yield of 31.18 mg/L was achieved under optimal conditions containing 53.6 g/L glycerol, 100 g/L soy peptone, and 10 g/L K2HPO4. A maximal yield of 40.96 mg/L MK-7 and a secretion ratio of 61.1% were obtained when 20 g/L soybean oil was supplemented at the logarithmic phase. The non-ionic surfactant span 20 was the second most promising surfactant in improving product yield, whereas addition of 2 g/L betaine exerted a minimal effect on secretion ratio of MK-7 at 19.1%. The results collectively showed that the supplementation of surfactants was an effective strategy to regulate cytomembrane permeability. Graphical abstract ᅟ.