Tumor lymphangiogenesis correlates with lymph node metastasis and clinicopathologic parameters in oral squamous cell carcinoma
Tumor lymphangiogenesis correlates with lymph node metastasis and clinicopathologic parameters in oral squamous cell carcinoma
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DOI：
10.1002/cncr.22900
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发表时间：
2007-09-15
期刊：
影响因子：
6.2
通讯作者：
Semba, Ichiro
中科院分区：
文献类型：
--
作者：
Miyahara, Mayumi;Tanuma, Jun-ichi;Semba, Ichiro
背景。淋巴管密度（LVD）和微血管密度（MVD）是评估肿瘤恶性潜能和患者生存的重要参数。在本报告中，作者将LVD定义为单位面积组织中d2 -40阳性淋巴管的密度，MVD定义为单位面积组织中cd105阳性微血管的密度。先前有报道称血管内皮生长因子C （VEGF-C）是LVD和MVD的主要调节剂。本研究的目的是阐明LVD和MVD在口腔鳞状细胞癌（OSCC）中的临床和预后意义，并阐明肿瘤组织中VEGF-C的淋巴管生成和血管生成活性。总共110个OSCC组织样本使用免疫组织化学方法评估LVD、MVD和VEGF-C的表达。检查这些参数与临床病理因素的相关性。与VEGF-C不表达或弱表达的肿瘤相比，VEGF-C非常高表达的肿瘤的LVD明显更高。LVD与淋巴结转移相关（P < 0.001）。MVD与淋巴结转移阳性相关（P < 0.001），而与VEGF-C表达无关。相反，VEGF-C的高表达与晚期肿瘤状态显著相关（P = 0.041）。高LVD （P < 0.001）、高MVD （P = 0.0028）和高VEGF-C表达（P = 0.048）患者的生存率较低。淋巴管生成主要影响无转移生存。目前的结果表明，在决定OSCC患者的治疗策略方面，LVD比MVD和VEGF-C更有用。
BACKGROUND. Lymphatic vessel density (LVD) and microvessel density (MVD) are important parameters for assessing the malignant potential of tumors and patient survival. In this report, the authors defined LVD as the density of D2-40-positive lymphatic vessels and MVD as the density of CD105-positive microvessels per unit area of tissue. It was reported previously that vascular endothelial growth factor C (VEGF-C) is a major modulator of LVD and MVD. The objectives of this study were to clarify the clinical and prognostic significance of both LVD and MVD in oral squamous cell carcinoma (OSCC) and to elucidate the lymphangiogenic and angiogenic activities of VEGF-C in cancer tissues.METHODS. in total, 110 OSCC tissue samples were evaluated for LVD, MVD, and expression of VEGF-C using immunohistochemistry. Correlations among these parameters and clinicopathologic factors were examined.RESULTS. LVD was significantly higher in tumors that had very high expression of VEGF-C compared with tumors that had no/weak expression of VEGF-C. LVD correlated well with lymph node metastasis (P < .001). MVD was correlated significantly with positive lymph node metastasis (P < .001) but not with VEGF-C expression. In contrast, high expression of VEGF-C was correlated significantly with advanced tumor status (P = .041). Survival rates were lower in patients who had higher LVD (P < .001), higher MVD (P = .0028), and strong VEGF-C expression (P = .048).CONCLUSIONS. Lymphangiogenesis predominantly influenced metastasis-free survival. The current results suggested that LVD is a more useful tool than MVD and VEGF-C for deciding on therapeutic strategies in patients with OSCC.