Superiority of Visipaque (lodixanol)-Controlled Density Gradient Over Ficoll-400 in Adult Porcine Islet Purification
Superiority of Visipaque (lodixanol)-Controlled Density Gradient Over Ficoll-400 in Adult Porcine Islet Purification
复制标题
Visipaque（洛地沙醇）控制密度梯度在成年猪胰岛纯化中优于 Ficoll-400
DOI：
10.1016/j.transproceed.2010.01.068
复制
发表时间：
2010-06-01
影响因子：
0.9
通讯作者：
Bo, W.
中科院分区：
文献类型：
--
作者：
Min, T.;Yi, L.;Bo, W.
Objective.胰岛是否具有足够和良好的生物学功能是阻碍异种移植的主要问题。本研究的目的是评估使用改良的Visipaque（碘克沙醇）和Ficoll-400纯化成年猪胰岛对收获、纯度、活力和功能的影响。根据纯化方法将12只成年猪胰腺随机分为碘克沙醇-威斯康星州（UW）组和Ficoll-400-UW组。采用胶原酶消化法分离猪胰腺。分离纯化后，双硫腙染色法检测胰岛产量和纯度，体外胰岛素释放试验和体内异种胰岛移植检测胰岛功能。纯化前胰岛产量无显著差异（5254.67 +/- 189.44 IEQ/g vs 5092.67 +/- 178.94 IEQ/g，P > 0.05）。纯化后，碘克沙醇-UW组收获的胰岛显著多于Ficoll-400-UW组：4222.00 +/- 228.84 IEQ/g对比3036.83 +/- 79.60 IEQ/g（P <0.05）。两组胰岛均表现出良好的胰岛素分泌能力。两组糖尿病大鼠胰岛存活时间无显著差异：8.2 +/- 1.619天vs 6.9 +/- 1.197天（P > 0.05）。改进的碘克沙醇-UW密度梯度法在提高纯化成年猪胰岛的数量、活力和胰岛素分泌方面上级Ficoll-400法，尽管其益处并未改善体内存活。
Objective. Sufficient and favorable biological functions of islets are major problems hindering xenotransplantation. The aim of the present study was to evaluate the effects on harvesting, purity, viability, and function of using improved Visipaque (iodixanol) and Ficoll-400 for adult porcine islet purification.Methods. Twelve adult porcine pancreata were randomly divided into an Iodixanol-University of Wisconsin (UW) group and a Ficoll-400-UW group according to the purification method. Porcine pancreata were isolated by collagenase digestion. After isolation and purification, the islet yield and purity were evaluated by dithizone staining, and islet function assessed by in vitro insulin release assays and in vivo islet xenotransplantation.Results. There were no marked differences in the islet yield before purification (5254.67 +/- 189.44 IEQ/g vs 5092.67 +/- 178.94 IEQ/g, P > .05). After purification, there were significantly more islets harvested in Iodixanol-UW group than in the Ficoll-400-UW group: 4222.00 +/- 228.84 IEQ/g vs 3036.83 +/- 79.60 IEQ/g (P < .05). Islets from the two groups showed satisfactory insulin secretory ability. There were no significant differences in islet survival times between the two groups in diabetic rats: 8.2 +/- 1.619 days vs 6.9 +/- 1.197 days (P > .05).Conclusion. The improved iodixanol-UW density gradient method was superior to Ficoll-400 method to improve the number, viability, and insulin secret of purified adult porcine islets although the benefits did not improve in vivo survival.