ADENINE POOL CATABOLISM IN THE ISCHEMIC, THE CALCIUM-DEPLETED ISCHEMIC, AND THE SUBSTRATE-FREE ANOXIC ISOLATED RAT-HEART - RELATIONSHIP TO CONTRACTURE DEVELOPMENT
ADENINE POOL CATABOLISM IN THE ISCHEMIC, THE CALCIUM-DEPLETED ISCHEMIC, AND THE SUBSTRATE-FREE ANOXIC ISOLATED RAT-HEART - RELATIONSHIP TO CONTRACTURE DEVELOPMENT
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DOI：
10.1016/s0022-2828(84)80039-5
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发表时间：
1984-01-01
影响因子：
5
通讯作者：
SEELYE, RN
中科院分区：
文献类型：
--
作者：
HUMPHREY, SM;HOLLISS, DG;SEELYE, RN
比较了遭受全缺血、低钙全缺血和全无底物缺氧的离体大鼠心脏的心肌腺嘌呤和次黄嘌呤库的代谢变化。在0和60分钟之间的时间间隔，使用脑室提取物的单独等分试样通过反相高效液相色谱(HPLC)测定组织pH或腺嘌呤池的成分及其分解代谢物。通过高氯酸[ClHO4]中和收集从缺氧灌注心脏排出的冠状动脉灌注液，并通过HPLC进行色谱分析。记录两组心脏左心室静息张力（挛缩）的发展情况。缺血60分钟后，主要分解代谢物（AMP、肌苷和次黄嘌呤）占总池的70%（分别为11、7和4μmol/g干重）。在同一时期的缺氧之后，从冠状动脉灌注液中回收了总池的50%，包括大致相等比例的腺苷、肌苷、次黄嘌呤和尿酸。组织中残留的主要产物是IMP和较小程度的AMP（分别为8和5μmol/g干重）。三组心脏中左心室挛缩的发生率不同，但始终与腺嘌呤池分解代谢的最大速率密切相关。在评估具有显着残余血流的局部缺血心肌中发生的生化事件时，可能应考虑组织中成分的损失以及缺氧灌注期间发生的从腺苷到 IMP 产生的途径的分歧。
Metabolic changes in the myocardial adenine and hypoxanthine pools of isolated rat hearts subjected to global ischemia, hypocalcemic global ischemia and global substrate-free anoxia were compared. At time intervals between 0 and 60 min separate aliquots of extracts of the ventricles were used to determine either tissue pH, or the components of the adenine pool and their catabolites by reverse phase high performance liquid chromatography (HPLC). The coronary perfusate draining from anoxically perfused hearts was collected over perchloric acid [ClHO4] neutralized and chromatographed by HPLC. The development of left ventricular resting tension (contracture) was recorded in the 2 groups of hearts. After 60 min ischemia the major catabolites, (AMP, inosine and hypoxanthine) comprised 70% of the total pool (11, 7 and 4 .mu.mol/g dry wt, respectively). After the same period of anoxia 50% of the total pool, comprise adenosine, inosine, hypoxanthine and uric acid in approximately equal proportions, was recovered from the coronary perfusate. The major products remaining in the tissue were IMP and, to a lesser extent AMP (8 and 5 .mu.mol/g dry wt, respectively). Left ventricular contracture developed at different rates in the 3 groups of hearts but always correlated closely with the maximum rate of adenine pool catabolism. The loss of components from the tissue and the divergence in pathway from adenosine to IMP production which occurs during anoxic perfusion should possibly be considered when assessing the biochemical events occurring in regionally ischemic heart muscle with significant residual flow.