Peptide P7 inhibits the bFGF-stimulated proliferation and invasion of SKOV3 cells
Peptide P7 inhibits the bFGF-stimulated proliferation and invasion of SKOV3 cells
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DOI：
10.3892/etm.2019.7309
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发表时间：
2019-04-01
影响因子：
2.7
通讯作者：
Qu, Wanglei
中科院分区：
文献类型：
--
作者：
Chen, Qiong;Yang, Ziying;Qu, Wanglei
肽P7与碱性成纤维细胞生长因子（bFGF）特异性结合，抑制多种类型癌细胞的增殖和侵袭。然而，这种作用仍有待于在卵巢癌来源的细胞系中得到证实。本研究利用蛋白P7治疗bfgf刺激的SKOV3上皮性卵巢癌细胞，探索P7的治疗潜力。MTT法和划伤法分别评价P7对bfgf刺激的SKOV3细胞增殖和迁移的影响。采用逆转录-定量聚合酶链反应方法检测尿激酶型纤溶酶原激活物（uPA）、基质金属肽酶(MMP)-2和-9基因表达，这些基因在细胞迁移/侵袭中起作用。不同浓度P7对SKOV3细胞形态和增殖无显著影响。而P7对bfgf刺激的SKOV3细胞的增殖和迁移有明显的抑制作用。与对照组相比，P7治疗显著降低了uPA、MMP-2和MMP-9的基因表达。总之，目前的结果表明，P7至少部分通过抑制bFGF起作用，可能在上皮性卵巢癌中具有潜在的治疗应用。
Peptide P7 specifically binds with basic fibroblast growth factor (bFGF) to inhibit the proliferation and invasion of numerous types of cancer cell. However, this effect has remained to be demonstrated in ovarian cancer-derived cell lines. In the present study, the protein P7 was used treat bFGF-stimulated SKOV3 epithelial ovarian cancer cells to explore the therapeutic potential of P7. An MTT and a scratch wound assay were used to respectively evaluate the proliferation and migration of bFGF-stimulated SKOV3 cells treated with P7. Reverse transcription-quantitative polymerase chain reaction analysis was used to detect the gene expression of urokinase-type plasminogen activator (uPA), as well as matrix metallopeptidase (MMP)-2 and -9, which have a role in cell migration/invasion. The morphology and proliferation of SKOV3 cells were not significantly affected by different concentrations of P7. However, P7 had an obvious inhibitory effect on the proliferation and migration of bFGF-stimulated SKOV3 cells. Treatment with P7 significantly lowered the gene expression of uPA, MMP-2 and MMP-9 compared with that in the control group. In conclusion, the present results suggested that P7, which, at least in part, acts through inhibition of bFGF, may have a potential therapeutic application in epithelial ovarian cancer.