Effect of blood serum, caffeine and heparin on in vitro phagocytosis of frozen-thawed bull sperm by neutrophils derived from the peripheral blood of cows
Effect of blood serum, caffeine and heparin on in vitro phagocytosis of frozen-thawed bull sperm by neutrophils derived from the peripheral blood of cows
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DOI：
10.1016/j.theriogenology.2010.03.019
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发表时间：
2010-09-01
期刊：
影响因子：
2.8
通讯作者：
Funahashi, H.
中科院分区：
文献类型：
--
作者：
Li, J. -C.;Funahashi, H.
尽管多形核白细胞(PMN)被招募到子宫腔内吞噬精子，但控制PMN吞噬能力的因素在牛身上还没有很好的文献报道。目的是确定血清、咖啡因和肝素对中性粒细胞对精子的趋化作用和中性粒细胞吞噬精子的影响。从奶牛的外周血中提取(离心法)多形核白细胞。在实验1中，无论有无精子存在，新鲜血清(血清1226个/mm(2)与牛血清白蛋白1110个/mm(2))对中性粒细胞趋化活性均有显著影响(P<0.01)，而热灭活血清(1099个/mm(2))对中性粒细胞的趋化活性无明显影响(P=0.65)。在新鲜血清存在下，中性粒细胞对活精子和死精子的吞噬作用均增加(P<；0.01)，但添加1 mM咖啡因的刺激作用降低(P<；0.01)(20.6-30.3%)。血清刺激的中性粒细胞趋化活性(1218个/mm(2))在咖啡因(1090个/mm(2))的存在下也降低(P<；0.01)。此外，在有血清的情况下补充肝素可降低中性粒细胞的吞噬能力(从43.8%降至21.5-31.7%)和趋化活性(从1124降至1048-1108个/mm(2))(P<；0.01)。我们推测，在活性补体存在下的调理作用可刺激中性粒细胞的吞噬和趋化活性，咖啡因和肝素均可降低血清刺激的中性粒细胞的吞噬和趋化活性。(C)2010 Elsevier Inc.保留所有权利。
Although polymorphonuclear leukocytes (PMNs) are recruited into the uterine lumen to phagocytize sperm, factors controlling the phagocytotic ability of PMNs in cattle are not well documented. The objective was to determine the effects of blood serum, caffeine, and heparin on chemotaxis of PMNs for sperm and phagocytosis of sperm by PMNs in cows. Polymorphonuclear leukocytes were obtained (centrifugation) from a cow's peripheral blood. In Experiment 1, the chemotactic activity of PMNs increased (P < 0.01) when fresh serum was included in the medium (1226 cells/mm(2) in serum vs. 1110 cells/mm(2) in BSA), regardless of the presence of sperm, whereas heat-inactivated serum (1099 cells/mm(2)) did not affect their activity (P = 0.65). Phagocytosis of live and dead sperm by PMNs both increased (P < 0.01) in the presence of fresh serum (incidences of 54.5 and 48.0%, respectively), but stimulation was decreased (P < 0.01) by supplementation of the medium with >= 1 mM caffeine (20.6-30.3%). Serum-stimulated chemotactic activity of PMNs (1218 cells/mm(2)) was also decreased (P < 0.01) in the presence of caffeine (1090 cells/mm(2)). Furthermore, supplementation of PMNs with heparin in the presence of serum decreased (P < 0.01) both phagocytotic (from 43.8% to 21.5-31.7%) and chemotactic activities of PMNs (from 1124 to 1048-1108 cells/mm(2)). We inferred that opsonization in the presence of active complement stimulated phagocytotic and chemotactic activities of PMNs, and that both caffeine and heparin decreased serum-stimulated phagocytotic and chemotactic activities of PMNs. (c) 2010 Elsevier Inc. All rights reserved.