Basal lamina formation by epidermal cells in cell culture.
Basal lamina formation by epidermal cells in cell culture.
复制标题
细胞培养物中表皮细胞形成基底层。
DOI：
10.1159/000396288
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
S. Taniguchi
中科院分区：
文献类型：
--
作者：
T. Hirone;S. Taniguchi
用电子显微镜观察了表皮细胞培养中基膜的形成。表皮细胞在未涂覆或涂覆有胶原凝胶的塑料皿上培养。细胞在塑料基质和胶原凝胶上生长、分层。而塑料表面上的培养物在与塑料基质的界面处未显示半桥粒和基底层的出现，胶原凝胶表面上的培养物显示半桥粒的发育，导致基底层在胶原凝胶和基底细胞的基底质膜的界面处的局灶性出现。此后，基膜发生在除了基底质膜的半桥粒区域之外的半桥粒间区域之下，通过连接形成连续层。
The formation of basal lamina in epidermal cell cultures was studied by electron microscopy. Epidermal cells were cultured on plastic dishes which were either uncoated or coated with collagen gel. The cells grew and stratified on the plastic substratum as well as on the collagen gel. While the cultures on the plastic surface showed no occurrence of hemidesmosomes and basal lamina at the interface with the plastic substratum, the cultures on the surface of the collagen gel showed development of hemidesmosomes leading to the focal occurrence of basal lamina at the interface of the collagen gel and the basal plasma membrane of basal cells. Thereafter, basal lamina occurred under the interhemidesmosomal areas in addition to the hemidesmosomal areas of the basal plasma membrane, forming a continuous layer by joining.